L. L. Kirkemo

Chemoproteomics reveals immunogenic and tumor-associated cell surface substrates of ectokinase CK2α

The paper uses chemoproteomics to identify immunogenic cell surface substrates of CK2α in the tumor microenvironment, showing that extracellular phosphorylation can generate immunogenic antigens.

PVRL2 Suppresses Anti-tumor Immunity Through PVRIG- and TIGIT-Independent Pathways

This study shows PVRL2 suppresses anti-tumor immunity independently of PVRIG and TIGIT, with deletion reducing tumor growth more than PD-L1 loss.

Site-specific proximity labeling at single residue resolution for identification of protein partners in vitro and on cells

This paper optimizes a high-resolution proximity labeling method to identify protein partners of HER2 on cells and in vitro, finding 20 high-confidence neighbors enriched compared to non-specific labeling.

Secreted HLA Fc-Fusion Profiles Immunopeptidome in Hypoxic PDAC and Cellular Senescence

This paper introduces a novel sHLA Fc-fusion method to identify HLA-associated peptides in hypoxic PDAC and senescence, revealing >30,000 unique peptides, many previously unreported.

Cell-surface tethered promiscuous biotinylators enable small-scale surface proteomics of human exosomes

This paper introduces two methods for rapid, small-scale cell surface biotinylation using tethered enzymes, enabling surface proteome analysis of cells and exosomes with high coverage and quantification of 1,020 surface proteins.

Trimeric SARS-CoV-2 Spike interacts with dimeric ACE2 with limited intra-Spike avidity

This study investigates the interaction between SARS-CoV-2 Spike and ACE2, showing that dimeric ACE2 binds with high affinity to Spike and blocks viral entry, with limited intra-Spike avidity.

Pharmacological complementation remedies an inborn error of lipid metabolism

This study tests two pharmacological strategies to enhance sobetirome's efficacy in lowering VLCFAs in X-ALD mice, finding that Sob-AM2 improves CNS VLCFA reduction but is limited by turnover kinetics.

Broad and thematic remodeling of the surface glycoproteome on isogenic cells transformed with driving proliferative oncogenes

This study uses proteomics to show how six oncogenes remodel the surface glycoproteome of breast cells, with common functional themes reversed by MEK inhibition.