Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology
Channabasavaiah B. Gurumurthy, Aidan R. O’Brien, Rolen M. Quadros, John Adams, Pilar Alcaide, Shinya Ayabe, Johnathan L. Ballard, Surinder K. Batra, M.-C. Beauchamp, Kathleen A. Becker, Guillaume Bernas, David Brough, Francisco Javier Carrillo-Salinas, Wesley Chan, Hanying Chen, , Atsushi Yoshiki
The paper re-evaluates the efficiency of a CRISPR-Cas9 method for generating conditional knockout alleles in mice, finding very low success rates and high rates of undesired alleles.
Genome biology · Aug 25, 2019Peer-reviewedGenetics