M. Johansson

Imaging single particle profiler to study nanoscale bioparticles using conventional confocal microscopy

The paper introduces iSPP, an imaging-based method for analyzing nanoscale bioparticles using conventional confocal microscopy and provides software for its application.

Anabolic sensitivity in healthy, lean, older men is associated with higher expression of amino acid sensors and mTORC1 activators

This study shows that lean, healthy older men maintain anabolic sensitivity to amino acids and exercise, with increased mTORC1 signaling and muscle protein synthesis, suggesting aging alone does not cause anabolic resistance.

A microfluidic platform for in situ studies of bacteria electroporation

This paper introduces a microfluidic platform for single-cell electroporation with in situ microscopy and demonstrates DNA delivery into bacteria, highlighting key factors affecting electroporation efficiency.

Dynamic binding of the bacterial chaperone Trigger factor to translating ribosomes

This study uses single-particle tracking to show that Trigger factor binds to translating ribosomes in E. coli with two distinct modes and competes with SRP for RNC binding.

Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization

This paper develops a method for sub-millisecond 3D tracking of single molecules in E. coli using cross-entropy minimization and achieves high precision with a new PSF positioning technique.

Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome Segregation

This study shows that TopoII's Chromatin Tether domain binds methylated histone H3K27me3 to prevent anaphase ultra-fine bridges, ensuring proper chromosome segregation.

Mechanism of DNA surface exploration and operator bypassing during target search

This study uses SMCT-FCS to show LacI slides on DNA, bypassing target sequences by hopping out of the DNA groove, revealing a speed-accuracy trade-off in target search.

More than a flying syringe: Using functional traits in vector borne disease research

This paper presents a framework for incorporating vector functional trait variation into disease transmission models to better understand and predict vector-borne disease dynamics.

Inferring rates of metastatic dissemination using stochastic network models

This paper develops a stochastic network model to infer metastatic dissemination rates using clinical data from tongue and ovarian cancer, revealing site-specific dissemination rates and enabling patient-specific metastasis predictions.

Elimination of ribosome inactivating factors improves the efficiency of Bacillus subtilis and Saccharomyces cerevisiae cell-free translational systems

This study shows that disrupting genes encoding ribosome inactivating factors improves the activity of cell-free translational systems in Bacillus subtilis and Saccharomyces cerevisiae.

Direct observation of rotation-coupled protein diffusion along DNA on the microsecond timescale

This study measures the rotation-coupled diffusion of LacI along DNA, finding it moves ~50 base pairs per revolution, supporting a facilitated diffusion model.