M. K. Lemberg

Rhomboid protease RHBDL4 promotes retrotranslocation of aggregation-prone proteins for degradation

This study identifies RHBDL4 as a rhomboid protease that promotes retrotranslocation of aggregation-prone proteins for degradation, working with the erlin ERAD complex.

Cleavage of PGAM5 by the intramembrane protease PARL is governed by transmembrane helix dynamics and oligomeric state

This study identifies key residues and structural features in PGAM5 that determine its cleavage by PARL, showing that membrane potential uncoupling affects its oligomeric state and processing.

Intramembrane protease SPP defines a cholesterol-regulated abundance control of the mevalonate pathway enzyme SQS

The paper shows that SPP cleaves SQS at high cholesterol levels, targeting it for degradation via TRC8, thereby regulating mevalonate pathway abundance.

Insights into the catalytic properties of the mitochondrial rhomboid protease PARL

This study investigates the catalytic properties of the mitochondrial rhomboid protease PARL using in vitro assays, revealing substrate preferences and activity enhancements under specific conditions.

CRISPR-Cas12a-assisted PCR tagging of mammalian genes

This paper describes a method for efficient endogenous gene tagging in mammalian cells using CRISPR-Cas12a and PCR cassettes, achieving up to 60% tagging efficiency with selection.

Intramembrane protease RHBDL4 cleaves oligosaccharyltransferase subunits to target them for ER-associated degradation

This study identifies RHBDL4 as a regulator of the oligosaccharyltransferase complex through ERAD-mediated degradation, revealing a novel link between ERAD and glycosylation regulation.

The metastable XBP1u transmembrane domain defines determinants for intramembrane proteolysis by signal peptide peptidase

The paper identifies a transmembrane domain in XBP1u that mediates ER membrane insertion and shows that SPP-catalyzed proteolysis depends on TM helix dynamics and side chain interactions.