M. Way

Branched actin networks mediate macrophage-dependent host microbiota homeostasis.

This study shows that Arpc5-containing Arp2/3 complexes are critical for macrophage function and maintaining host-microbiota homeostasis, as their loss leads to intestinal inflammation.

CK-666 and CK-869 differentially inhibit Arp2/3 iso-complexes

This study shows CK-666 and CK-869 differentially inhibit Arp2/3 iso-complexes, with CK-869 uniquely affecting ArpC1B and Arp3-containing complexes, impacting phagocytosis and migration in macrophages.

Regeneration of actin filament branches from the same Arp2/3 complex

The paper shows that Arp2/3 complex can re-nucleate actin branches without NPF activation after debranching, suggesting a self-repair mechanism for actin networks.

Cortactin stabilizes actin branches by bridging activated Arp2/3 to its nucleated actin filament

This study reveals how cortactin stabilizes actin branches by interacting with activated Arp3 at the branch site, displacing other nucleation promoting factors.

Kinesin-1 transports morphologically distinct intracellular virions during vaccinia infection

This study shows kinesin-1 transports two distinct vaccinia virion forms, IMV and IEV, with different speeds and motor complex numbers, and demonstrates the importance of kinesin-1 for viral spread.

The level of Nck rather than N-WASP determines the rate of actin-based motility of Vaccinia

The paper quantifies viral and host proteins involved in Vaccinia virus actin-based motility, finding that Nck levels, not N-WASP, determine virus movement speed.

ARPC5 deficiency leads to severe early onset systemic inflammation and early mortality

This study identifies ARPC5 deficiency as a cause of severe early-onset systemic inflammation and early mortality in humans and mice.

Biomimetic actin cortices shape cell-sized lipid vesicles

This study reconstitutes biomimetic actin cortices inside lipid vesicles to show how actin polymerization forces can both rigidify and deform membranes depending on nucleator distribution.

Cryo-ET of infected cells reveals that a succession of two lattices drives vaccinia virus assembly

This study uses cryo-ET to show that vaccinia virus maturation involves a new pseudohexagonal lattice that controls virion shape and dimensions.

Nucleation and stability of branched versus linear Arp2/3-generated actin filaments

This study compares the nucleation and stability of branched versus linear actin filaments generated by Arp2/3 complex activation by VCA domains or SPIN90, revealing similar regulatory responses but distinct mechanical and temporal stability.

ARPC5 Isoforms and Their Regulation by Calcium-Calmodulin-N-WASP Drive Distinct Arp2/3-dependent Actin Remodeling Events in CD4 T Cells

The paper identifies distinct roles of ARPC5 isoforms in regulating Arp2/3-dependent actin remodeling in CD4 T cells under different stimuli.

The Relative Binding Position of Nck and Grb2 Adaptors Dramatically Impacts Actin-Based Motility of Vaccinia Virus

The study shows that specific spatial arrangements of pTyr motifs in viral proteins are necessary for efficient actin-based motility and spread of Vaccinia virus.

MICAL2 acts through Arp3B isoform-specific Arp2/3 complexes to destabilize branched actin networks

The paper shows MICAL2 regulates Arp3B-specific Arp2/3 complexes to control branched actin network disassembly through oxidation of Met293.

Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation

This study shows that lamellipodin stabilizes protrusions and promotes adhesion formation, enhancing cell migration efficiency.

Cryo-EM of human Arp2/3 complexes provides structural insights into actin nucleation modulation by ARPC5 isoforms

The paper determines cryo-EM structures of human Arp2/3 complexes with different ARPC5 isoforms to show how isoform variation affects complex activity.

B cells extract antigens using Arp2/3-generated actin foci interspersed with linear filaments

This study identifies the role of Arp2/3-generated actin foci and formin-produced linear filaments in B cell antigen extraction through immune synapse mechanics.