Author

P. N. Hedde

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 2 Cell Biology· 2 Biophysics· 1 Microbiology· 1

Signature Assays, Organisms & Methods

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Approach:deep learning· 3 Approach:experimental assay· 3 Research Focus:cell type annotation· 2 Organism:human· 2 Assay:proteomics· 2 Approach:statistical model· 2 Research Focus:cell signaling & trafficking· 1 Research Focus:clustering· 1

Frequent Co-Authors

E. Gratton· 3 shared W. Zhao· 3 shared B. Barylko· 2 shared D. M. Jameson· 2 shared J. P. Albanesi· 2 shared J. Zimak· 2 shared A. Fung· 1 shared A. Ganesan· 1 shared

Activity by Year

2020-2021 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 16 mutual co-author links

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3 shared papersBioengineering

E. Gratton

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Author profile →

6 papers

Recent·Most cited

A mutation associated with Charcot-Marie-Tooth disease enhances the formation of stable dynamin 2 complexes in cells

P. N. Hedde, B. Barylko, C.-L. Chiu, J. P. Albanesi, D. M. Jameson, N. G. James

This study shows a CMT-causing DNM2 mutation, {Delta}DEE, fails to undergo auto-inhibition and forms cytoplasmic inclusions, similar to CNM mutants, challenging the current understanding of disease-specific mechanisms.

bioRxiv · Dec 13, 2021 · PreprintCell Biology

Differential mobility and self-association of Arc/Arg3.1 in the cytoplasm and nucleus of living cells

P. N. Hedde, B. Barylko, D. D. Binns, D. M. Jameson, J. P. Albanesi

This study uses fluorescence nanoimaging to show that Arc/Arg3.1 exists as monomers and dimers in the cytoplasm and is predominantly monomeric and more diffusive in the nucleus, moving freely between PML-NBs and nucleoplasm.

bioRxiv · Dec 2, 2021Cell Biology

A high throughput bispecific antibody discovery pipeline

A. I. Segaliny, J. Jayaraman, X. Chen, J. Chong, R. Luxon, A. Fung, Q. Fu, X. Jiang, R. Rivera, X. Ma, C. Ren, J. Zimak, P. N. Hedde, Y. Shang, G. Wu, W. Zhao

This paper introduces a high throughput single-cell pipeline for bispecific antibody discovery, demonstrating its efficiency in identifying rare functional clones and uncovering design principles for improved immunotherapeutics.

bioRxiv · Sep 7, 2021Bioengineering

Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis

T. Vu, A. Vallmitjana, J. Gu, K. La, Q. Xu, J. Flores, J. Zimak, J. Shiu, L. Hosohama, J. Wu, C. R. Douglas, M. Waterman, A. Ganesan, P. N. Hedde, E. Gratton, W. Zhao

This paper introduces MOSAICA, a spatial transcriptomics technology that combines spectral and lifetime encoding with machine learning to enable highly multiplexed in situ biomarker profiling.

bioRxiv · Jun 22, 2021Bioengineering

Ultrafast phasor-based hyperspectral snapshot microscopy for biomedical imaging

P. N. Hedde, R. Cinco, L. Malacrida, A. Kamaid, E. Gratton

This paper introduces ultrafast phasor-based hyperspectral snapshot microscopy for biomedical imaging, achieving 10-100x faster imaging speeds and enabling metabolic imaging of live 3D mouse tissues.

bioRxiv · Oct 14, 2020 · PreprintBiophysics

A Modular Microarray Imaging System for Highly Specific COVID-19 Antibody Testing

P. N. Hedde, T. J. Abram, A. Jain, R. Nakajima, R. Ramiro de Assis, T. Pearce, A. Jasinskas, M. N. Toosky, S. Khan, P. L. Felgner, E. Gratton, W. Zhao

The paper introduces a low-cost, portable imaging system for specific COVID-19 antibody testing using microarrays, achieving performance comparable to expensive commercial readers.

bioRxiv · May 22, 2020Microbiology