K. M. Dean

Mechanically Sheared Axially Swept Light-Sheet Microscopy

The paper introduces a new image acquisition method for light-sheet microscopes that improves spatial context placement and reduces post-processing needs, achieving higher field of view and resolution.

navigate: an open-source platform for smart light-sheet microscopy.

navigate provides an open-source platform for smart light-sheet microscopy, enabling automated and user-friendly LSFM workflows without requiring specialized expertise.

Keratin isoform shifts modulate motility signals during wound healing

The paper shows that keratin isoform shifts during wound healing modulate motility signals by activating myosin motors through isoform-specific interactions with vimentin shuttling kinases.

Resolution doubling in light-sheet microscopy via oblique plane structured illumination

This paper demonstrates isotropic resolution doubling in light-sheet microscopy using oblique plane structured illumination, achieving sub-150nm lateral resolution with reduced phototoxicity and faster volumetric imaging.

Increasing the Field-of-View in Oblique Plane Microscopy via optical tiling

This paper introduces a dual-axis scan unit for oblique plane microscopy to increase field-of-view and imaging speed, enabling high-resolution volumetric imaging of zebrafish embryos.

Compression-dependent microtubule reinforcement comprises a mechanostat which enables cells to navigate confined environments

The paper shows that microtubules form a mechanostat to coordinate contractility and nuclear positioning during confined cell migration.

Blebs Promote Cell Survival by Assembling Oncogenic Signaling Hubs

This study shows blebs form signaling hubs that promote anoikis resistance in cancer cells by recruiting septins and activating ERK/PI3K pathways.

Heterozygous mutation of Vegfr3 decreases renal lymphatics but is dispensable for renal function

This study shows that heterozygous Vegfr3 mutation reduces renal lymphatics but does not impair renal function or histology, even after cisplatin injury.

u-track 3D: measuring and interrogating dense particle dynamics in three dimensions.

u-track 3D provides a software package for analyzing dense 3D particle dynamics in microscopy data by improving visualization, trajectory validation, and interactive exploration.

Proteolysis-free cell migration through crowded environments via mechanical worrying

The paper shows amoeboid melanoma cells migrate through crowded environments by mechanically degrading extracellular matrix via bleb-driven abrading, without proteolysis.

Real-Time Multi-Angle Projection Imaging of Biological Dynamics

This paper introduces a projection imaging method that enables real-time, multi-angle visualization of biological processes with reduced data overhead.

A systematic and quantitative comparison of lattice and Gaussian light-sheets

This study compares lattice and Gaussian light-sheets quantitatively, finding they are similar in thickness, confocal parameter, and propagation length.

A Versatile Oblique Plane Microscope for Large-Scale and High-Resolution Imaging of Subcellular Dynamics.

This paper introduces an improved oblique plane microscope with enhanced resolution and usability, demonstrating its application in imaging various subcellular dynamics and challenging biological phenomena.

Converting lateral scanning into axial focusing to speed up 3D microscopy

This paper introduces an optical design that converts lateral scanning into axial focusing, enabling rapid, high-resolution 3D imaging with axially swept light-sheet microscopy.

Direct Arp2/3-vinculin binding is essential for cell spreading, but only on compliant substrates and in 3D

The paper investigates the role of actin-binding proteins in cell spreading on 2D and 3D substrates, finding that Arp2/3-vinculin binding is critical for 3D spreading but not 2D.

Universal Light-Sheet Generation with Field Synthesis

This paper introduces Field Synthesis, a theorem that simplifies light-sheet generation for microscopy, enabling high-throughput, multicolor imaging with reduced photobleaching.