D. Segal

Specificity and tunability of efflux pumps: a new role for the proton gradient?

This study shows that efflux pump specificity is influenced by proton gradient and membrane potential, offering new strategies to combat bacterial resistance.

Imaging of cellular dynamics in vitro and in situ: from a whole organism to sub-cellular imaging with self-driving, multi-scale microscopy

The paper introduces a self-driving, multi-resolution light-sheet microscope to simultaneously observe sub-cellular dynamics and whole organisms over extended periods, applying it to study development, cancer, and immune interactions in zebrafish.

Structured RhoGEF recruitment drives myosin II organization on large exocytotic vesicles

The paper investigates how Rho1 and RhoGEF2 regulate actomyosin organization during exocytosis in Drosophila, revealing a biphasic mechanism for membrane crumpling.

Pervasive mislocalization of pathogenic coding variants underlying human disorders

The paper develops a high-throughput imaging platform to assess the functional impact of missense variants on protein localization, finding that mislocalization is common in pathogenic variants.

Unique features of the gut microbiome characterized in animal models of Angelman Syndrome

This study characterizes gut microbiome changes in AS animal models, identifying specific bacterial groups associated with GI symptoms and Ube3a maternal deletion.

In vivo profiling of site-specific human cancer cell states in zebrafish

The paper uses zebrafish xenografts and computer vision to study how Ewing Sarcoma cell morphology changes in different sites and under EWSR1-FLI1 expression variations.

Formation and disassembly of a contractile actomyosin network mediates content release from large secretory vesicles

The paper identifies an actin assembly and disassembly cycle in Drosophila salivary gland vesicles that drives secretion by coordinating Rho1 signaling and actin dynamics.