S. Dean

Unlocking Trypanosome Biology: A Comprehensive Protein-Tagging Toolkit for Localization and Functional Analysis

The paper expands a protein-tagging toolkit for trypanosomes with over 100 vectors, enabling flexible localization and functional analysis of proteins using diverse fluorescent and biochemical tags.

Subcellular protein localisation of Trypanosoma brucei bloodstream form-upregulated proteins maps stage-specific adaptations

The paper maps subcellular localisation of bloodstream form-upregulated proteins in Trypanosoma brucei, revealing stage-specific organelle associations.

Genome-wide subcellular protein localisation in the flagellate parasite Trypanosoma brucei

The paper maps subcellular protein localization in Trypanosoma brucei using fluorescence microscopy, revealing functional insights and organelle adaptations.

Polo-like kinase 4 homodimerization is not required for catalytic activation, autodestruction, or centriole assembly

This study shows that Plk4 homodimerization is not required for catalytic activation, autodestruction, or centriole assembly, as dimer mutants remain functional through concentration-dependent aggregation.

Monoallelic antigen expression in trypanosomes requires a stage-specific transcription activator

The paper identifies ESB1 as a stage-specific activator required for monoallelic VSG transcription in Trypanosoma brucei, revealing its role in Pol I recruitment to the expression site body.

Bro1 stimulates Vps4 activity to promote Intralumenal Vesicle Formation during Multivesicular Body biogenesis

This study shows that the Bro1 V domain stimulates Vps4 activity to promote ESCRT-III-driven intralumenal vesicle formation during MVB biogenesis, coordinating cargo sorting with membrane remodeling.

APEX2 proximity proteomics resolves flagellum subdomains and identifies flagellum tip-specific proteins in Trypanosoma brucei

This paper uses APEX2 proximity proteomics to identify flagellum subdomain-specific proteins in Trypanosoma brucei, revealing tip-specific proteins involved in signal transduction.

A key regulatory protein for flagellum length control in stable flagella

This paper identifies Cep164C as a key protein involved in maintaining stable flagellum length in Trypanosoma brucei by acting as a molecular lock at the flagellum base.

Measuring the impact of an interdisciplinary learning project on nursing, architecture and landscape design students empathy

This paper evaluates an interdisciplinary learning project's impact on empathy levels of nursing, architecture, and landscape design students toward women and children experiencing homelessness and domestic violence.

Basalin: an evolutionary unconstrained protein revealed via a conserved role in basal plate function

The paper identifies basalin, a conserved protein essential for basal plate function in trypanosomes, and suggests it may act as an interaction hub with divergent orthologs across species.