S. Vaughan

Plasmodium NEK1 coordinates MTOC organisation and kinetochore attachment during rapid mitosis in male gamete formation

This study identifies NEK1's role in organizing the MTOC and ensuring kinetochore attachment during rapid mitosis in Plasmodium male gamete formation.

The C-terminus of CFAP410 forms a tetrameric helical bundle that is essential for its localization to the basal body

This study determines the structure of CFAP410's C-terminal domain and shows that its tetrameric helical bundle is essential for basal body localization, explaining how the L224P mutation causes ciliopathies.

3D electron microscopy of the Leishmania mexicana cell cycle: Patterns of organelle duplication and segregation and their implications for parasite biology

This study uses 3D electron microscopy to analyze Leishmania mexicana cell cycle stages, revealing organelle duplication, segregation patterns, and metabolic adaptations in different environments.

Identification of 30 transition fibre proteins reveals a complex and dynamic structure with essential roles in ciliogenesis

The paper identifies 30 new transition fibre proteins in Trypanosoma brucei and shows their dynamic roles in ciliogenesis and flagellum length regulation.

Atypical flagella assembly and haploid genome coiling during male gamete formation in Plasmodium

This study uses electron microscopy to reveal the 3D organization of axonemes and genome coiling during male gamete formation in Plasmodium berghei.

Unlocking Trypanosome Biology: A Comprehensive Protein-Tagging Toolkit for Localization and Functional Analysis

The paper expands a protein-tagging toolkit for trypanosomes with over 100 vectors, enabling flexible localization and functional analysis of proteins using diverse fluorescent and biochemical tags.

CFAP410 has a bimodular architecture with a conserved surface patch on its N-terminal LRR motif for binding interaction partners

This study reports the crystal structure of the N-terminal domain of CFAP410 and shows how mutations in this domain disrupt its structural stability and interaction with partners, leading to disease.

Genome-wide subcellular protein localisation in the flagellate parasite Trypanosoma brucei

The paper maps subcellular protein localization in Trypanosoma brucei using fluorescence microscopy, revealing functional insights and organelle adaptations.

Plasmodium SAS4/CPAP is a flagellum basal body component during male gametogenesis but is not essential for parasite transmission

This study investigates the role of SAS4/CPAP in Plasmodium male gametogenesis, showing it is part of the basal body but not essential for gamete formation or parasite transmission.

Cellular electron tomography of the apical complex in the apicomplexan parasite Eimeria tenella shows a highly organised gateway for regulated secretion.

This study uses electron tomography to reveal the 3D structure of the apical complex in Eimeria tenella, showing organized trafficking and secretion mechanisms.

Genome-wide functional analysis reveals key roles for kinesins in the mammalian and mosquito stages of the malaria parasite life cycle

The paper identifies key kinesins involved in the malaria parasite life cycle in mammalian and mosquito hosts, showing kinesin-13 is essential for growth in mammals while others are critical for mosquito transmission.

Protein Phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages

The paper investigates the role of Protein Phosphatase 1 (PP1) in atypical mitotic and meiotic divisions during Plasmodium sexual stages, suggesting PP1 inhibitors could block parasite transmission.

A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of Plasmodium berghei during gametogony and parasite transmission.

This study identifies CRK5 as a critical regulator of atypical DNA replication in Plasmodium berghei during gametogony and mosquito transmission.

Control of assembly of extra-axonemal structures: the paraflagellar rod of trypanosomes

The paper identifies two cytoplasmic proteins essential for paraflagellar rod formation in trypanosomes, highlighting the multi-compartmental coordination of extra-axonemal structure assembly.

A key regulatory protein for flagellum length control in stable flagella

This paper identifies Cep164C as a key protein involved in maintaining stable flagellum length in Trypanosoma brucei by acting as a molecular lock at the flagellum base.

Kinesin-8B controls basal body function and flagellum formation and is key to malaria parasite transmission

This study identifies kinesin-8B's role in malaria parasite flagellum formation and transmission by analyzing its localization and function during gamete development.

Bidirectional intraflagellar transport is restricted to two sets of microtubule doublets in the trypanosome flagellum

This study identifies two distinct IFT tracks in trypanosome flagella using microscopy, showing bidirectional transport on parallel lines and dynamic conversion of anterograde to retrograde trains.

A molecular model of the mitochondrial genome segregation machinery in Trypanosoma brucei

The paper uses super-resolution microscopy and biochemical analysis to show the hierarchical assembly of the TAC in Trypanosoma brucei, independent of the mitochondrial genome.