B. Bothner

Cryo-EM captures the coordination of long-range allostery and asymmetric electron transfer by a bi-copper cluster in the nitrogenase-like DPOR complex

The paper uses Cryo-EM to reveal how asymmetry and allostery coordinate long-range electron transfer in the DPOR enzyme through a bi-copper cluster.

Wrapping of single-stranded DNA by Replication Protein A and modulation through phosphorylation

This study uses single-molecule techniques to show that RPA partially wraps ssDNA and that phosphorylation at Ser-384 modulates this wrapping.

The metabolome of individuals with knee osteoarthritis is influenced by 18-months of an exercise and weight loss intervention and sex: the IDEA trial

The paper examines how 18-months of diet, exercise, or combined interventions affect the serum metabolome of knee OA patients, revealing sex-specific metabolic changes.

Metabolomic Profiles and Pathways in Osteoarthritic Human Cartilage: A Comparative Analysis with Healthy Cartilage

This study compares metabolomic profiles of healthy and osteoarthritic human cartilage to identify disease-associated metabolic endotypes and pathways.

Homodecameric Rad52 promotes single-position Rad51 nucleation in homologous recombination

This study reveals that homodecameric Rad52 facilitates single-position Rad51 nucleation in homologous recombination through specific structural and interaction features.

Metabolomic Phenotypes Reflect Patient Sex and Injury Status: A Cross-Sectional Analysis of Human Synovial Fluid

This study identifies distinct metabolomic profiles in synovial fluid associated with knee injury types and patient sex, suggesting metabolic differences linked to post-traumatic osteoarthritis development.

An Aurora B-RPA signaling axis secures chromosome segregation fidelity

This study identifies a mitosis-specific regulation of RPA by Aurora B kinase, showing that phosphorylation of RPA70 at Ser-384 is essential for chromosome segregation fidelity and genomic stability.

Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunit.

This study identifies key residues in eIF6 and RPL23 that mediate their interaction and shows how mutations in SDS disrupt this interaction, offering a therapeutic target for cancer and SDS.

Rtt105 configurationally staples RPA and blocks facilitated exchange and interactions with RPA-interacting proteins

This study shows Rtt105 binds to RPA, staples it, and blocks RIP interactions without ssDNA, while stabilizing RPA-ssDNA filaments with ssDNA.

Core Protein-Directed Antivirals and Importinβ Can Synergistically Disrupt HBV Capsids

This study shows that HAP12, a CpAM antiviral, synergizes with importinβ to disrupt HBV capsids, revealing an unexpected mechanism of action involving host protein-induced catalysis.

The cortical bone metabolome of C57BL/6J mice is sexually dimorphic

The study compares metabolomes of cortical bone, whole bone, and bone marrow in C57BL/6J mice, revealing sex differences and associations with bone strength.

Hydrogen-deuterium exchange reveals a dynamic DNA binding map of Replication Protein A

This study uses HDX-MS to analyze the dynamic DNA binding properties of RPA domains, revealing that DBD-C is most involved in stabilizing RPA on ssDNA.

Primary human chondrocytes respond to compression with plasma membrane receptors and by microtubule activation: a phosphoproteomic study

This study identifies phosphoproteomic responses of osteoarthritic chondrocytes to compression, revealing microtubule and calcium signaling activation.