N. Pokhrel

Fluorescent human RPA to track assembly dynamics on DNA

This paper describes fluorescently modified human RPA to study its dynamic interactions with ssDNA and other proteins.

Homodecameric Rad52 promotes single-position Rad51 nucleation in homologous recombination

This study reveals that homodecameric Rad52 facilitates single-position Rad51 nucleation in homologous recombination through specific structural and interaction features.

An Aurora B-RPA signaling axis secures chromosome segregation fidelity

This study identifies a mitosis-specific regulation of RPA by Aurora B kinase, showing that phosphorylation of RPA70 at Ser-384 is essential for chromosome segregation fidelity and genomic stability.

HREM, RNAseq and cell-cycle analyses reveal the role of the G2/M-regulatory protein, Wee1, on the survivability of chicken embryos during diapause.

This study uses HREM, RNAseq, and molecular assays to show that Wee1 upregulation during cold diapause arrests the cell cycle, preserving embryonic structure and enabling resumption of development.

Rtt105 configurationally staples RPA and blocks facilitated exchange and interactions with RPA-interacting proteins

This study shows Rtt105 binds to RPA, staples it, and blocks RIP interactions without ssDNA, while stabilizing RPA-ssDNA filaments with ssDNA.

Hydrogen-deuterium exchange reveals a dynamic DNA binding map of Replication Protein A

This study uses HDX-MS to analyze the dynamic DNA binding properties of RPA domains, revealing that DBD-C is most involved in stabilizing RPA on ssDNA.

Dynamics and Selective Remodeling of the DNA Binding Domains of RPA

The paper visualizes RPA's DNA binding domain dynamics and shows how Rad52 modulates DBD-D to enable ssDNA remodeling during homologous recombination.