S. Marciano

RBD-based high affinity ACE2 antagonist limits SARS-CoV-2 replication in upper and lower airways

RBD-62, a high-affinity ACE2 antagonist, limits SARS-CoV-2 replication in both upper and lower airways in rhesus macaques without blocking memory responses or causing anti-drug immunity.

Reversing protonation of weakly basic drugs greatly enhances intracellular diffusion and decreases lysosomal sequestration

Modulation of type I interferon responses potently inhibits SARS-CoV-2 replication and inflammation in rhesus macaques

This study shows that modulating type I interferon responses in rhesus macaques reduces SARS-CoV-2 viral load and inflammation, suggesting a potential therapeutic strategy for severe COVID-19.

Protein Quaternary Structures in Solution are a Mixture of Multiple forms

The paper assesses the oligomeric states of 17 bacterial proteins using multiple techniques, finding that most exist in multiple forms and that AlphaFold Multimer better predicts their quaternary structures than existing databases.

SARS-CoV-2 RBD in vitro evolution follows contagious mutation spread, yet generates an able infection inhibitor

The paper shows that in vitro evolved SARS-CoV-2 RBD mutations, like S477N, E484K, and N501Y, correlate with increased infectivity and are inhibited by high-affinity RBD drugs.

An enhanced yeast display platform demonstrates the binding plasticity under various selection pressures

This paper enhances a yeast display platform with improved protein stability and fluorescence, demonstrating binding plasticity under different selection pressures.

Line-FRAP, a versatile method based on fluorescence recovery after photobleaching to measure diffusion rates in vitro and in vivo

Line-FRAP measures diffusion rates in vitro and in vivo using confocal microscopy, showing slower diffusion in cells compared to buffer and osmotic pressure effects.