S. Petry

Mechanism of how the universal module XMAP215 γ-TuRC nucleates microtubules

This study identifies how XMAP215 interacts with γ-TuRC to promote microtubule nucleation through specific TOG domains and reveals structural insights into their cooperative function.

Building on-chip cytoskeletal circuits via branched microtubule networks

This paper develops a microfluidic platform to engineer microtubule circuits by integrating microfabrication with microtubule branching reactions, enabling controlled MT architecture for on-chip applications.

Impact of structural sampling, coupling scheme and state of interest on the energy transfer in CP29

The paper evaluates how different coupling schemes and structural sampling affect energy transfer efficiency in CP29, finding significant differences in B band EET depending on the method used.

RanGTP regulates the augmin complex

The paper shows that RanGTP directly regulates the augmin complex, which is a spindle assembly factor, to control branching microtubule nucleation during mitosis.

The conserved centrosomal motif, γTuNA, forms a dimer that directly activates microtubule nucleation by the γ-tubulin ring complex (γTuRC).

This study identifies γTuNA as a dimer that directly activates γTuRC to nucleate microtubules, opposing stathmin inhibition.

Acentrosomal spindles assemble from branching microtubule nucleation near chromosomes

The paper shows that chromosomes can nucleate microtubules to form spindles without centrosomes, using branching nucleation near kinetochores.

Confinement size determines the architecture of Ran-induced microtubule networks

This study shows that the architecture of Ran-induced microtubule networks depends on droplet size and Ran concentration in Xenopus egg extracts.

Branching microtubule nucleation is controlled by importin-mediated inhibition of TPX2 phase separation

This study shows that importin-mediated interactions inhibit TPX2 phase separation, thereby regulating branching microtubule nucleation during spindle assembly.

The transition state and regulation of γ-TuRC-mediated microtubule nucleation revealed by single molecule microscopy

This study uses single molecule microscopy to reveal how γ-TuRC nucleates microtubules, showing a cooperative mechanism involving 4 β-tubulin dimers and the role of XMAP215 in enhancing nucleation efficiency.

Biochemical reconstitution of branching microtubule nucleation

This study reconstitutes branching microtubule nucleation using purified Xenopus proteins, identifying augmin and TPX2 as essential factors that guide gamma-TuRC to nucleate branched microtubules.

Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation

The paper shows TPX2 phase separates with tubulin to enhance and spatially coordinate microtubule nucleation in vitro and in cytosol.

Spatiotemporal organization of branched microtubule networks

The paper studies branched microtubule network formation in Xenopus egg extracts, showing that TPX2, augmin, and γ-TuRC regulate nucleation through a sequential reaction pathway.