This study fuses histone variants to Cas9 to enhance homology-directed repair (HDR) while suppressing non-homologous end joining (NHEJ), finding that H2A.1 improves the HDR/NHEJ ratio better than H2A.X.

bioRxiv · Jul 2, 2023 · PreprintCell Biology

Evidence of the simultaneous replications of active viruses in specimens positive for multiple respiratory viruses

M. Kawase, R. Suwa, S. Sugimoto, M. Kakizaki, Y. Kume, M. Chishiki, T. Ono, H. Okabe, S. Norito, M. Ujike, M. Hosoya, K. Hashimoto, K. Shirato

The paper confirms simultaneous replication of multiple respiratory viruses in low-copy-number specimens using HBTEC-ALI cultures.

bioRxiv · Apr 26, 2023Microbiology

A mechanism of melanogenesis mediated by E-cadherin downregulation and its involvement in solar lentigines

D. Inoue, T. Narita, K. Ishikawa, K. Maeno, A. Motoyama, T. Ono, H. Aoki, T. Shibata

This study identifies E-cadherin downregulation as a mechanism driving melanogenesis in age spots and shows that Rosa multiflora extract can upregulate E-cadherin in keratinocytes.

bioRxiv · Jan 9, 2023Cell Biology

In Vivo Delivery of Therapeutic Molecules by Transplantation of Genome-Edited Induced Pluripotent Stem Cells

I. Nakajima, T. Tsukimura, T. Ono, T. Shiga, H. Shitara, T. Togawa, H. Sakuraba, Y. Miyaoka

This study shows genome-edited iPSCs can secrete therapeutic molecules to treat Fabry disease in mice.

bioRxiv · Jan 3, 2023 · PreprintCell Biology

NF-κB Decoy ODN-Loaded Poly lactic-co-glycolic Acid Nanospheres Inhibit Alveolar Ridge Resorption

A. C. S. Huang, Y. Ishida, K. Li, D. Rintanalert, K. Hatano-sato, S. Oishi, J. Hosomichi, R. Usumi-Fujita, H. Yamaguchi, H. Tsujimoto, A. Sasai, A. Ochi, H. Watanabe, T. Ono

This study investigates the therapeutic effect of NF-κB decoy ODN-loaded PLGA nanospheres in reducing alveolar ridge resorption after tooth extraction in rats.

bioRxiv · Aug 30, 2022Immunology

Myogenetic oligodeoxynucleotide (myoDN) complexed with berberine promotes differentiation of chicken skeletal muscle myoblasts

Y. Nihashi, S. Shinji, K. Umezawa, T. Shimosato, T. Ono, H. Kagami, T. Takaya

This study shows that myogenetic oligodeoxynucleotides (myoDNs) enhance chicken myoblast differentiation, with the iSN04-berberine complex showing increased activity in myotube formation and myogenic gene expression.

bioRxiv · Dec 19, 2020Cell Biology

Lipopolysaccharide preconditioning augments phagocytosis of malaria-parasitized red blood cells through induced bone marrow-derived macrophages in the liver, thereby increasing the murine survival after Plasmodium yoelii infection

T. Ono, Y. Yamaguchi, H. Nakashima, M. Nakashima, T. Ishikiriyama, S. Seki, M. Kinoshita

This study shows that LPS preconditioning enhances phagocytic clearance of malaria parasites by liver macrophages, improving survival in mice infected with Plasmodium yoelii.

bioRxiv · May 22, 2020Immunology

Lab-on-a-graphene-FET detection of key molecular events underpinning influenza virus infection and effect of antiviral drugs

T. Ono, K. Kamada, R. Hayashi, A. R. Piacenti, C. Gabutt, N. Sriwilaijaroen, H. Hiramatsu, Y. Kanai, K. Inoue, S.-i. Nakakita, T. Kawahara, Y. Ie, Y. Watanabe, Y. Suzuki, S. Contera, K. Matsumoto

This study uses graphene-FETs to detect molecular events during influenza infection and monitor antiviral drug effects in real-time.

bioRxiv · Mar 18, 2020 · PreprintBioengineering

Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms

M. Takagi, T. Ono, T. Natsume, C. Sakamoto, M. Nakao, N. Saitoh, M. T. Kanemaki, T. Hirano, N. Imamoto

This study shows Ki-67 and condensins have independent yet cooperative roles in maintaining mitotic chromosome structure through distinct mechanisms.

bioRxiv · Oct 12, 2017Cell Biology