T. M. Lohman

Subunit Communication within Dimeric SF1 DNA Helicases

This study shows that specific dimer interactions and ATPase activity in both subunits are required for helicase activity in E. coli Rep and UvrD, with subunit communication demonstrated through Trp fluorescence.

E. coli RecBCD Nuclease Domain Regulates Helicase Activity but not Single Stranded DNA Translocase Activity

This study shows that the nuclease domain of E. coli RecBCD affects dsDNA unwinding rates but not ssDNA translocation, suggesting the nuclease domain influences base pair melting rather than translocation.

Mycobacterium tuberculosis Ku stimulates multi-round DNA unwinding by UvrD1 monomers

This study shows that Mtb Ku stimulates UvrD1 monomers to unwind DNA in multiple rounds, revealing a mechanism for DNA repair activation in mycobacteria.

Helicase Activity Promoted Through Dynamic Interactions Between a ssDNA Translocase and a Diffusing SSB Protein

The paper shows that Pif1 helicase uses its translocase activity to push RPA along ssDNA, disrupting duplex DNA and demonstrating a new mechanism for directional DNA unwinding.

How Glutamate Promotes Liquid-liquid Phase Separation and DNA Binding Cooperativity of E. coli SSB Protein

This study shows that potassium glutamate promotes liquid-liquid phase separation and non-nearest-neighbor cooperativity of E. coli SSB protein through amide group interactions, while KCl inhibits these processes.

Mycobacterium tuberculosis DNA-repair helicase UvrD1 is activated by redox-dependent dimerization via a conserved 2B domain cysteine.

This study shows that Mtb UvrD1 dimerizes under oxidative conditions, enhancing its DNA unwinding activity through a conserved cysteine in the 2B domain.

Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase

This study investigates the DNA unwinding mechanism of UvrD, a non-hexameric helicase, revealing a stepwise translocation with variable base pair steps and strand sequestration.

Probing E. coli SSB Protein-DNA topology by reversing DNA backbone polarity

This study investigates how E. coli and P. falciparum SSB proteins bind ssDNA with reversed backbone polarity, finding that both maintain strict polarity preference but adapt their wrapping topology.

Comparative analysis of CPI-motif regulation of biochemical functions of actin capping protein

The paper compares the biochemical functions of CPI-motif-containing peptides from different protein families and identifies sequence variations affecting their interactions with actin capping protein.

A Novel Antenna Protein Complex in the Life Cycle of Cyanobacterial Photosystem II

The paper identifies a novel PSII subcomplex lacking reaction center proteins, suggesting a protective mechanism for PSII repair in cyanobacteria.