G. Pigino

Structure, interaction, and nervous connectivity of beta cell primary cilia

This study reveals the structural and spatial organization of beta cell primary cilia in mouse and human islets, showing their interactions with neighboring cells and nerves, and their role in islet connectivity.

The Tubulin Nano-Code: a protofilament-specific pattern of tubulin post-translational modifications regulates ciliary beating mechanics

The paper identifies protofilament-specific tubulin post-translational modification nano-patterns that regulate ciliary beating by interacting with axonemal dyneins and nexin-dynein complexes.

Efficient precision genome editing of Chlamydomonas reinhardtii with CRISPR/Cas

This paper develops an efficient method for scarless genome editing in Chlamydomonas reinhardtii using CRISPR/Cas, enabling precise knock-in mutagenesis without prior mutant lines.

Integrative modeling reveals the molecular architecture of the Intraflagellar Transport A (IFT-A) complex

The paper models the structure of the IFT-A complex using experimental and computational methods, revealing insights into ciliopathy-associated mutations.

In situ architecture of the ciliary base reveals the stepwise assembly of IFT trains

The paper visualizes the native structure of the ciliary transition zone and the stepwise assembly of IFT trains in Chlamydomonas cells.

Intraflagellar transport trains can turn around without the ciliary tip complex

This study shows that intraflagellar transport trains can turn around without a dedicated calcium-dependent tip complex in Chlamydomonas reinhardtii.

A WDR35-dependent coatomer transports ciliary membrane proteins from the Golgi to the cilia

The paper shows WDR35 is part of a coatomer complex that transports ciliary membrane proteins from the Golgi to cilia, essential for cilia elongation.

Reorganization of budding yeast cytoplasm upon energy depletion

This study uses electron tomography to show that yeast cells reorganize their cytoplasm and form membrane-less organelles when ATP is depleted, as part of a survival strategy.

Microridges are apical projections formed of branched F-actin networks that organize the glycan layer

The paper identifies microridges as apical projections composed of branched F-actin networks that organize the glycan layer in zebrafish epidermal cells.

Filament formation by the translation factor eIF2B regulates protein synthesis in starved cells

This study shows that eIF2B filament formation in starved yeast cells regulates translation by acidification-induced assembly, independent of Gcn2 kinase, promoting stress survival and recovery.