Paul Guichard

This study identifies DCX as essential for microtubule branching in Plasmodium falciparum gametocytes, which is required for mosquito transmission, independent of its role in the conoid.

This study evaluates gene supplementation of MYO7A via dual AAVs and CRISPR/Cas activation of Myo7b in animal models and human retinal organoids to treat Usher syndrome 1B.

This study investigates the role of CCDC40 in trypanosome flagellum structure and function, revealing that its depletion causes structural defects but not impaired cell division.

The paper introduces HAK-actin, a probe for imaging actin cytoskeleton compatible with U-ExM, showing robust staining across human cells, microbial eukaryotes, and mouse retinal tissue.

This study provides a molecular and structural map of the ciliary base in mammalian multiciliated cells using advanced imaging and molecular techniques.

This study uses cryo-expansion microscopy to visualize the nanoscale architecture of immune synapses in T cells and their interactions with tumor cells, revealing structural details of signaling molecules and lytic granules.

The paper investigates how centrosome architecture and m6A-dependent regulation influence p53 surveillance after whole-genome doubling, identifying key molecular mechanisms.

This study identifies C2CD3 as a radial organizer of the distal centriole and appendages, revealing its role in structuring the luminal ring and interacting with the DISCO complex.

Ultrastructure expansion microscopy applied to 221 cultured planktonic eukaryotes maps diverse microtubule and centrin-based cytoskeletons, including dinoflagellate apical centrin rings and cryptophyte cortical centrin meshes.

The paper identifies A-C linker proteins and shows their role in centriole cohesion, duplication, and structure.

This study identifies SSNA-1 as a centriole constituent required for structural integrity and bipolar spindle assembly in C. elegans.

The paper uses cell-free assays to show PACRG and FAP20 synergistically stabilize microtubule doublets by reducing B-tubule depolymerization and increasing rescue frequency.

This study shows that glutamylation imbalance in photoreceptor cells leads to structural defects and degeneration, highlighting its role in maintaining outer segment architecture.

This study identifies a phospho-regulatory module involving PPP2R3C and MAP3K1 that regulates centrosome function and is linked to congenital syndromes with gonadal dysgenesis.

This study fine-tunes FAM161A gene therapy in mice to restore retinal function by optimizing promoter activity, dosage, and isoform delivery.

This study identifies bi-allelic CCDC146 mutations as a cause of non-syndromic male infertility in humans and mice, showing its role in sperm flagellum structure and microtubule organization.

This study uses expansion microscopy to investigate LCA5-related photoreceptor defects and evaluates gene therapy efficacy in a mouse model.

This study uses expansion microscopy to reveal the cytoskeleton organization in malaria parasites, showing the conservation of a conoid structure previously thought to be absent.

This paper introduces UltraExM, a method that enables subdiffraction-resolution imaging of preserved ultrastructures using standard optical microscopy, revealing new insights into centriolar structures.