H. Stahlberg

Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution

This paper applies low-dose cryo-EM and 4D-STEM ptychography to achieve sub-nanometer resolution 3D reconstructions of frozen hydrated proteins.

Identification of a Binding Site for Small Molecule Inhibitors Targeting Human TRPM4

The paper identifies the binding site of small molecule inhibitors NBA and IBA in TRPM4 using cryo-EM and patch clamp experiments.

ACE2 mimetic antibody potently neutralizes all SARS-CoV-2 variants and fully protects in XBB.1.5 challenged monkeys

Structure of a membrane-bound menaquinol:organohalide oxidoreductase

The paper presents the first cryo-EM structure of a functional menaquinol:organohalide oxidoreductase from Desulfitobacterium hafniense, revealing its mechanism of electron transfer and energy conservation during organohalide respiration.

Cryo-EM structures and binding of mouse and human ACE2 to SARS-CoV-2 variants of concern indicate that mutations enabling immune escape could expand host range

The paper reports cryo-EM structures of mouse ACE2 bound to SARS-CoV-2 VOC Spike proteins, showing mutations enabling immune escape may expand host range.

De novo design of site-specific protein interactions with learned surface fingerprints

This paper designs novel protein interactions using geometric deep learning to generate surface fingerprints, achieving nanomolar affinity for four targets through computational and experimental methods.

SARS-CoV-2 Omicron potently neutralized by a novel antibody with unique Spike binding properties

This paper identifies a potent human monoclonal antibody, P2G3, that neutralizes multiple SARS-CoV-2 variants including Omicron, and demonstrates its protective efficacy in a monkey model.

Structural analysis of the Spike of the Omicron SARS-COV-2 variant by cryo-EM and implications for immune evasion.

The paper reports cryo-EM structures of mouse ACE2 bound to Spike proteins of four SARS-CoV-2 variants, revealing mutations that enhance binding and immune evasion.

Cryo-EM structure of native human thyroglobulin

The paper presents the cryo-EM structure of human thyroglobulin, revealing its 3D structure and providing insights into hormone synthesis and glycosylation sites.

Structural and molecular determinants for the interaction of ExbB from Serratia marcescens and HasB, a TonB paralog

The paper determines the cryo-EM structures of ExbBSm and ExbB-ExbDSm complex, identifies key residues for function and interaction with TonB/HasB, and reveals the role of ExbBSm extension in heme acquisition.

Insight into Lipopolysaccharide Translocation by Cryo-EM structures of a LptDE Transporter in Complex with Pro-Macrobodies

The paper uses cryo-EM to determine structures of the LptDE transporter complex with Pro-Macrobodies, revealing partially and fully opened states of the transporter during LPS translocation.

Structures of ABCG2 under turnover conditions reveal a key step in drug transport mechanism

This study presents cryo-EM structures of ABCG2 under turnover conditions, revealing conformational changes that elucidate the transport mechanism and substrate specificity.

Structural basis of drug recognition by the multidrug transporter ABCG2

This study presents cryo-EM structures of ABCG2 bound to anticancer drugs, revealing how it recognizes and binds exogenous compounds and the structural basis for drug-induced ATPase activity.

Structural basis of the activation of the CC chemokine receptor 5 by a chemokine agonist

The paper reports the cryo-EM structure of CCR5 bound to a chemokine agonist and Gi protein, revealing its activation mechanism.

Cryo-EM structural studies of the agonist complexed human TRPV4 ion-channel reveals novel structural rearrangements resulting in an open-conformation

This paper reports the cryo-EM structure of hTRPV4 in an open conformation induced by 4-PDD agonist, revealing molecular mechanisms of activation and providing a template for inhibitor design.

Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoprotein

This study uses cryo-EM to show that hACE2 binding causes SARS-CoV-2 Spike trimer disassembly and S1 domain rearrangement, suggesting a mechanism for soluble hACE2's therapeutic effect.

The architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography

This study uses cryo-electron tomography to reveal the architectural diversity of the centriole cartwheel region across four species, showing structural variations in periodicity and organization.

Determinants for forming a supramolecular myelin-like proteolipid lattice

This study uses structural and functional analyses to reveal how myelin protein P2 forms a supramolecular lattice between lipid bilayers, identifying key residues and interactions involved in membrane binding.

Retrieving High-Resolution Information from Disordered 2D Crystals by Single Particle Cryo-EM

The paper develops a method to improve resolution and resolve conformational heterogeneity in 2D crystals using single-particle refinement, achieving 4 Å resolution for the MloK1 potassium channel.

Microfluidic protein isolation and sample preparation for high resolution cryo-EM

This paper introduces a microfluidic method for isolating and preparing proteins for cryo-EM, enabling high-resolution structure determination from minimal cell lysate.

Cryo-EM structure of the rhodopsin-Gαi-βγ complex reveals binding of the rhodopsin C-terminal tail to the Gβ subunit

The paper presents a cryo-EM structure of the rhodopsin-Gαi-βγ complex, revealing the C-terminal tail of rhodopsin bound to the Gβ subunit and identifying common anchoring points across G protein-subtype interactions.

Molecular structure and function of myelin protein P0 in membrane stacking

This study characterizes the molecular structure and function of myelin protein P0, showing how its extracellular domains stack lipid membranes and stabilize the intraperiod line in peripheral myelin.

Focus: The interface between data collection and data processing in cryo-EM

Focus is a software package that streamlines cryo-EM data collection and processing by integrating data import, management, and real-time monitoring in a user-friendly environment.

Image processing techniques for high-resolution structure determination from badly ordered 2D crystals

The paper develops image-processing algorithms to improve 3D structure determination from badly ordered 2D crystals, achieving resolution improvements to approximately 5 Å.