W. Kukulski

The punctate localisation of the yeast sterol transporter Ysp2p is determined by three dimerisation interfaces in its C-terminus

Supramolecular architecture of the ER-mitochondria encounter structure in its native environment

The paper determines the supramolecular architecture of the ERMES complex in yeast using integrative structural biology methods, revealing its role in lipid transfer between ER and mitochondria.

Ultrastructure of COPII vesicle formation characterised by correlative light and electron microscopy.

This study uses correlative light and electron microscopy to examine COPII coat structure and its role in membrane remodeling during protein transport in yeast cells.

The architecture of Cidec-mediated interfaces between lipid droplets

The paper visualizes lipid droplet interfaces using cryo-ET and shows lipid transfer occurs through closely apposed monolayers with exponential kinetics, suggesting a pressure-driven mechanism.

Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion

This study reveals asymmetric plasma membrane conformations in yeast cell fusion, driven by turgor pressure and exocytosis/endocytosis ratios, which facilitate cell fusion.

The supramolecular landscape of growing human axons

This study uses advanced imaging techniques to reveal the molecular architecture of growing human axons in cerebral organoids, highlighting structural features and functional implications.

Cargo crowding drives sorting stringency in COPII vesicles

The paper shows that cargo crowding in COPII vesicles enhances sorting stringency by restricting bulk flow, improving ER quality control.

Closed mitosis requires local disassembly of the nuclear envelope

This study identifies a novel protein involved in local nuclear envelope breakdown during closed mitosis in fission yeast.

Multiplexed electron microscopy by fluorescent barcoding allows screening for ultrastructural phenotype

The paper introduces a method using fluorescent barcoding and electron microscopy to enable high-throughput screening of ultrastructural phenotypes in yeast.

Rod-shaped tricalbins contribute to PM asymmetry at curved ER-PM contact sites

The paper investigates how tricalbins in budding yeast regulate PM asymmetry at ER-PM contact sites through structural and genetic analyses.

Molecular and topological reorganizations in mitochondrial architecture interplay during Bax-mediated steps of apoptosis

This study investigates how Bax activity affects mitochondrial structure during apoptosis, revealing molecular and topological changes that contribute to outer membrane rupture.

The contributions of the actin machinery to endocytic membrane bending and vesicle formation

This study investigates how actin network components contribute to endocytic membrane bending and vesicle formation in yeast using genetic mutants and hybrid imaging.