M. Schuldiner

A proteome-wide yeast degron collection for the dynamic study of protein function.

The paper introduces an AID library for on-demand protein depletion in yeast, enabling systematic study of protein function and uncovering new roles in cell cycle and mitochondrial processes.

An unconventional interaction interface between the peroxisomal targeting factor Pex5 and Eci1 enables PTS1 independent import

The paper identifies an unconventional Pex5-Eci1 interaction interface that enables PTS1-independent peroxisomal import of Eci1 in yeast.

Mitochondrial inner membrane remodeling as a driving force of organelle shaping

This study investigates how mitochondrial inner membrane remodeling drives organelle shape changes, linking cristae formation to mitochondrial morphology and function.

The molecular mechanism of on-demand sterol biosynthesis at organelle contact sites

This study identifies Yet3 as a protein that recruits sterol biosynthesis enzymes to ER contact sites, enabling on-demand sterol production and influencing organellar structure and function.

PEX39 facilitates the peroxisomal import of PTS2 proteins

This study identifies PEX39 as a cytosolic peroxin that facilitates PTS2-protein import by interacting with PEX7 and stabilizing its binding to cargo.

Mpf1 is a novel factor that affects the dual distribution of tail-anchored proteins between mitochondria and peroxisomes

This study identifies Mpf1 as a novel factor influencing the dual targeting of tail-anchored proteins between mitochondria and peroxisomes in yeast.

A new targeting motif for endoplasmic reticulum surface proteins

The paper identifies an amphipathic helix as a targeting motif for ER surface proteins in yeast and humans, showing its conservation and role in membrane localization.

Profiling the LAM family of contact site tethers provides insights into their regulation and function

The paper profiles the LAM family of contact site tethers to understand their localization and function, revealing unique and shared roles through proximity labeling.

A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes

The paper uses a bi-genomic split-GFP assay to identify mitochondrial matrix and inner membrane proteins, revealing 50 previously unvisualized proteins and characterizing dually localized proteins.

The antifungal activity of cymoxanil is associated with proton pump inhibition and disruption of plasma membrane potential

This study investigates the antifungal mechanism of cymoxanil, finding it disrupts plasma membrane potential and inhibits proton pumps in yeast, suggesting a mode of action involving membrane transport and acidification.

A stable microtubule bundle formed through an orchestrated multistep process controls quiescence exit

The paper describes the molecular mechanism of microtubule bundle assembly and disassembly during quiescence exit in yeast.

A new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress

The paper introduces a method to isolate organellar membranes from yeast and uses it to study lipid composition and stress responses in the endoplasmic reticulum and vacuolar membranes.

Dually targeted proteins regulate proximity between peroxisomes and partner organelles

The paper investigates dually targeted proteins in yeast and shows they act as dynamic tethers between peroxisomes and partner organelles, contributing to their proximity and communication.

ER translocation of suboptimal targeting sequences depends on Sec61β/Sbh1 and its phosphorylation

The paper shows that phosphorylation of Sbh1 regulates ER translocation of proteins with suboptimal targeting sequences by inducing conformational changes.

A systematic approach to study protein-substrate specificity enables the identification of Ssh1 substrate range

The paper introduces Cel-lctiv, a method to study PPIs in yeast, and demonstrates its use in identifying the unique substrate range of Ssh1.

Determining the targeting specificity of the selective peroxisomal targeting factor Pex9

The paper investigates the targeting specificity of Pex9 by analyzing PTS1 motifs and structural features, finding that hydrophobicity of the amino acid before the PTS1 tripeptide determines its binding specificity.

Regulation of major bacterial survival strategies by transcript sequestration in a membraneless organelle

The paper shows that TmaR-mediated liquid-liquid phase separation in E. coli regulates sugar metabolism and enables bacterial survival strategies like motility and biofilm formation.

Systematic multi-level analysis of an organelle proteome reveals new peroxisomal functions

The paper identifies new peroxisomal functions in yeast by expanding the organellar proteome and analyzing sub-organellar localization and metabolic roles.

Systematic analysis of membrane contact sites in Saccharomyces cerevisiae uncovers modulators of cellular lipid distribution

The paper systematically analyzes membrane contact sites in yeast to identify new proteins involved in lipid distribution.

Identification of Pex34p as a component of the peroxisomal de novo biogenesis machinery in yeast

The paper identifies Pex34p as a crucial factor for de novo peroxisome biogenesis in yeast, alongside Pex25p, through genetic screening and interaction studies.

Mitochondrial ribosomal proteins developed unconventional mitochondrial targeting signals due to structural constraints

The paper identifies internal mitochondrial targeting signals in yeast mitochondrial ribosomal proteins, showing that 30% of them use non-conventional signals not reliably predicted by existing methods.

Translational control as a novel regulator of gradient sensing and chemotropism in yeast

This study identifies translational control as a regulator of chemotropism in yeast by discovering genes involved in gradient sensing and mating.

The mitochondrial surface receptor Tom70 protects the cytosol against mitoprotein-induced stress

The paper identifies Tom70's role in recruiting cytosolic chaperones to the mitochondrial outer membrane to mitigate proteotoxicity from hydrophobic precursor proteins.

Pex14p phosphorylation modulates import of citrate synthase 2 into peroxisomes in Saccharomyces cerevisiae

This study shows that phosphorylation of Pex14p at serine 266 regulates the import of Cit2p into peroxisomes, affecting yeast metabolism under different carbon sources.

AMPK regulates ESCRT-dependent microautophagy of proteasomes concomitant with proteasome storage granule assembly during glucose starvation

The paper shows AMPK and ESCRTs drive microautophagy of proteasomes during glucose starvation, leading to proteasome storage granule assembly and functional proteasome retention.

Multiplexed electron microscopy by fluorescent barcoding allows screening for ultrastructural phenotype

The paper introduces a method using fluorescent barcoding and electron microscopy to enable high-throughput screening of ultrastructural phenotypes in yeast.

Transfer of Septin Rings to Cytokinetic Remnants Directs Age-Sensitive ER stress Surveillance Cell Cycle Re-entry

Non-Canonical Regulation of Phosphatidylserine Metabolism by a Phosphatidylinositol Transfer Protein and a Phosphatidylinositol 4-OH Kinase

The paper shows that Psd2-dependent phosphatidylethanomaine production in yeast relies on Psd2, Sfh4, and Stt4, with Sfh4 activating the pathway through a physical interaction rather than its PtdIns transfer activity.

Cytotoxicity of 1-deoxysphingolipid Unraveled by Genome-wide Genetic Screens and Lipidomics

This study identifies cytotoxic effects of 1-deoxysphingolipid species and reveals mechanisms linking their accumulation to HSAN IA/C pathology through genetic screens and lipidomics.

Systematic assessment of GFP tag position on protein localization and growth fitness in yeast

The paper assesses the impact of GFP tag position on protein localization and yeast growth fitness using a competition-based approach.