Lewis Evans

This paper develops a genetic toolbox for tissue-derived human organoids, enabling efficient gene manipulation through CRISPR-based methods and flow cytometry.

The paper identifies LRRK2-regulated pathways for extracellular tau uptake in human neurons using CRISPR screens, showing LRRK2's role in monomeric tau endocytosis.

The paper constructs a single-cell atlas of the frontal cortex in early-onset monogenic Alzheimer's disease to identify cellular and molecular changes in disease mechanisms.

This study shows that tau mutations in FTD cause hyperphosphorylation, mislocalization, and nuclear membrane deformation, leading to defective nucleocytoplasmic transport.

The paper investigates how monomeric and aggregated tau enter human neurons through distinct pathways, identifying dynamin- and actin-dependent mechanisms for monomeric tau and dynamin-dependent clathrin-mediated endocytosis for aggregated tau.