Andreas M. Küffner

The paper reconstructs minimal pyrenoids using EPYC1 proteins to study their evolution and carbon concentration mechanisms.

The paper identifies Form IF Rubiscos that are highly active, specific, and function without the small subunit, challenging the necessity of subunit complexity in Rubisco evolution.

The paper investigates the evolutionary origin of chaperone dependence in Rubisco biogenesis and finds that this dependence evolved neutrally and can be reversed without affecting catalytic function.

This study shows that biomolecular condensates enhance enzymatic activity by buffering pH, allowing enzymes to function effectively in acidic conditions and enabling pH-dependent cascade reactions.

This paper presents a computational workflow for designing and testing E. coli metabolic sensors that detect glyoxylate and glycolate across a wide concentration range, with applications in bioengineering and environmental monitoring.