Timothy F. Shay

ESCRT-0 regulates AMPA receptor currents and Ca2+- dependent signaling

This study shows that Hrs, an ESCRT-0 protein, modulates AMPA receptor currents and Ca2+-dependent signaling by sensing and responding to neuronal activity at synapses.

A Perturb-seq screen guided by species divergence uncovers pathways for collateral artery formation

The paper uses Perturb-seq in mice to identify genes that promote collateral artery formation by comparing gene expression between guinea pigs and mice.

Structural basis of liver de-targeting and neuronal tropism of CNS-targeted AAV capsids

The paper solves cryo-EM structures of AAV capsids and their complexes with AAVR to design variants with reduced liver targeting and altered brain cell tropism.

Genetically encoded assembly recorder temporally resolves cellular histories in cellulo and in vivo

This paper introduces GEMINI, a tool that records cellular activity histories with high temporal resolution, and demonstrates its application in tracking transcriptional changes and inflammation dynamics in vivo.

Systemic AAV delivery of a calcium indicator in marmosets: functional validation in visual area MT

This study demonstrates that systemic AAV delivery enables widespread calcium indicator expression in marmosets, allowing functional optical imaging of visual area MT at multiple scales.

AAVs Targeting Human Carbonic Anhydrase IV Enhance Gene Delivery to the Brain

This study engineers AAVs targeting human carbonic anhydrase IV to enhance brain gene delivery, finding that optimized variants achieve 100-fold greater transduction than AAV9.

Adeno-Associated Viral Tools to Trace Neural Development and Connectivity Across Amphibians

This paper identifies AAV serotypes effective for transducing amphibian CNS cells and develops tools for neural circuit tracing and manipulation in multiple amphibian species.