Douglas S. Kim

The paper describes engineering eFRET voltage indicators with positive-going fluorescence responses by modifying microbial rhodopsins.

The paper introduces jYCaMP1, a calcium indicator optimized for two-photon imaging at wavelengths above 1000 nm, showing improved performance in mice and flies.

The paper presents transgenic mice expressing the red calcium indicator jRGECO1a for in vivo neuronal imaging, showing comparable or better signal-to-noise ratios than viral methods.

Structure-guided mutagenesis of GCaMP6 plus neuron-based screening yields a jGCaMP7 family, each variant tuned to a different regime: single spikes, neurites and neuropil, or population imaging.