Loren L. Looger

This paper reports the generation of stable transgenic dwarf cuttlefish expressing fluorescent proteins using CRISPR and transposons, enabling live imaging of cell dynamics during embryonic development.

The paper uses imaging and biochemical methods to analyze the effects of GCaMP overexpression on mammalian cells, identifying cellular morphology changes and cytoskeletal interactions.

The paper shows that astrocyte activity drives brainwide changes in sensory, integrative, and motor computations during a passive state in zebrafish.

The paper improves a GABA sensor, iGABASnFR2, with higher sensitivity and faster kinetics, enabling better in vivo imaging of GABAergic transmission.

GESIAP3.0 enables precise quantitative analysis of neuromodulatory synaptic transmission in behaving animals by correcting for tissue movement and optimizing synaptic information extraction.

This paper develops genetically encoded fluorescent sensors to measure polyamine levels, uptake, and distribution in cells, revealing compounds that modulate polyamine levels.

This study develops a fluorescent opioid biosensor to measure fentanyl levels in mice and links pharmacokinetics to behavioral patterns using computational ethology.

This paper presents a post-GWAS analysis pipeline to identify functional variants and target genes for SLE using integrative genomic and epigenomic data.

The paper uses a new GABA sensor to identify over 40 GABA-releasing neuron types in the mouse retina and shows how their spatial structure and release kinetics shape inhibitory signaling.

The paper uses fluorescent sensors to study glutamate and GABA dynamics during interictal spikes and seizures in a rodent model, revealing a progressive failure of inhibitory restraint leading to seizures.

This study uses a multi-core fiber optic imaging platform to show that astrocyte calcium activity in the cerebral cortex varies with internal motivational states and neuromodulatory systems.

This paper develops improved glutamate indicators with faster activation kinetics and better synaptic localization for imaging synaptic transmission in neurons.

The paper develops fast and sensitive GCaMP indicators for neural activity imaging, achieving ultra-fast kinetics and high sensitivity.

The paper shows that volume-transmitted GABA waves drive epileptiform rhythms in hippocampal networks by elevating tonic GABAA receptor conductance across multiple cells.

This study measures the temporal dynamics of SSRIs in different cellular compartments and shows they accumulate in membranes and organelles, suggesting new roles in therapeutic effects and discontinuation syndrome.

GESIAP is a MATLAB-based image analysis program that processes genetically encoded sensor data to analyze neuromodulator transmission properties across cell types and species.

The paper identifies fold-switching proteins by analyzing secondary structure propensities in the NusG family, finding 25% of sequences undergo α-helix ↔ β-sheet transitions.

The paper introduces a sequence-based method to predict fold-switching proteins that transition between α-helix and β-strand structures, achieving a Matthews Correlation Coefficient of 0.70.

The paper describes engineering eFRET voltage indicators with positive-going fluorescence responses by modifying microbial rhodopsins.

The paper introduces jYCaMP1, a calcium indicator optimized for two-photon imaging at wavelengths above 1000 nm, showing improved performance in mice and flies.

The paper identifies evolved fold switchers from amino acid sequences by analyzing inconsistencies in secondary structure predictions between helix and strand conformations.

iAChSnFR, a fluorescent indicator built from a bacterial periplasmic binding protein, reports acetylcholine with large, fast signals in mouse, fish, fly and worm preparations.

This study identifies Drosulfakinin as a regulator of social isolation-induced aggression in Drosophila, showing a U-shaped relationship between Dsk signaling and aggressive behavior.

This paper introduces a two-photon imaging technique that achieves high-speed, high-resolution imaging of neural activity in mice at over 1 kHz frame rates.

This paper develops a genetically encoded fluorescent sensor, iGABASnFR, to monitor GABA in vivo and demonstrates its use in tracking GABA dynamics in various neural contexts.

The paper identifies a functional RASGRP1 variant associated with SLE risk through epigenetic and experimental validation, showing it regulates RASGRP1 expression via hnRNP-K binding.

The paper uses multiplex imaging to simultaneously measure presynaptic Ca2+ and glutamate release at multiple synapses in situ.

The paper identifies fold-switching proteins by analyzing inconsistencies in secondary structure predictions from amino acid sequences.

The paper identifies two types of motor cortex neurons with distinct roles in movement preparation and execution using single-cell profiling and axonal reconstructions.