This paper develops a dual-channel whole-brain imaging system for Drosophila to simultaneously measure calcium and dopamine dynamics during walking, revealing distinct functional maps in specific brain regions.

bioRxiv · Jun 4, 2026 · PreprintNeuroscience

High-performance GFP-based calcium indicators for imaging activity in neuronal populations and microcompartments

Hod Dana, Yi Sun, Boaz Mohar, Brad K. Hulse, Jeremy P. Hasseman, Getahun Tsegaye, Arthur Tsang, Allan M. Wong, Ronak H. Patel, John J Macklin, Yang Chen, Arthur Konnerth, Vivek Jayaraman, Loren L. Looger, Eric R. Schreiter, , Douglas S. Kim

Structure-guided mutagenesis of GCaMP6 plus neuron-based screening yields a jGCaMP7 family, each variant tuned to a different regime: single spikes, neurites and neuropil, or population imaging.

bioRxiv · Oct 3, 2018 · PreprintNeuroscience

Sensitive red protein calcium indicators for imaging neural activity

Hod Dana, Boaz Mohar, Yi Sun, Sujatha Narayan, Andrew Gordus, Jeremy P Hasseman, Getahun Tsegaye, Graham T Holt, Amy Hu, Deepika Walpita, Ronak Patel, John J Macklin, Cornelia I Bargmann, Misha B Ahrens, Eric R Schreiter, , Douglas S Kim

The paper introduces improved red calcium indicators that match the sensitivity of GFP-based indicators, enabling better in vivo neural activity imaging.

eLife · Mar 24, 2016Peer-reviewedNeuroscience