Sakthi D. Moorthy

This study identifies a cis-regulatory enhancer cluster, SRR2-18, that regulates Sox2 transcription in mouse neural stem/progenitor cells, affecting their regional identity and differentiation into posterior neural fates.

The paper shows that Lefty1 and Lefty2 genes in mouse embryonic stem cells are regulated by separate enhancers despite being in the same TAD.

The paper shows that high affinity enhancer-promoter interactions can bypass CTCF/cohesin insulation and maintain gene expression in some tissues but not others.

The paper shows that transcriptional control and chromatin architecture at the Sox2 locus are decoupled, with distinct regulatory mechanisms.

The paper identifies a diversity threshold of transcription factor binding sites (TFBS) required for enhancer activity in embryonic stem cells, showing that a diverse set of TFBS can replace optimized regulatory sequences.