The paper develops a protocol to generate floxed alleles in zebrafish for cell-specific knockout, demonstrating its utility in studying gene function.

bioRxiv · Sep 6, 2022 · PreprintGenetics

5' Modifications Improve Potency and Efficacy of DNA Donors for Precision Genome Editing

Krishna Sumanth Ghanta, Zexiang Chen, Aamir Mir, Gregoriy A. Dokshin, Pranathi Meda Krishnamurthy, Yeonsoo Yoon, Judith Gallant, Ping Xu, Xiao-Ou Zhang, Ahmet Rasit Ozturk, Masahiro Shin, Feston Idrizi, Pengpeng Liu, Hassan Gneid, Alireza Edraki, , Craig C. Mello

This study shows that 5' modifications to DNA repair templates enhance precision genome editing efficiency in multiple animal models and human cells.

eLife · Oct 19, 2021Peer-reviewedMolecular Biology

VEGFC induced cell cycle arrest mediates sprouting and differentiation of venous and lymphatic endothelial cells

Ayelet Jerafi-Vider, Ivan Bassi, Noga Moshe, Yaara Tevet, Gideon Hen, Daniel Splittstoesser, Masahiro Shin, Nathan D. Lawson, Karina Yaniv

This study shows that VEGFC induces cell-cycle arrest in endothelial cells, promoting sprouting and differentiation in venous and lymphatic vessels.

Cell Reports · Jun 1, 2021Peer-reviewedDevelopmental Biology