Richard A. Stein

Sequence determinants of pathogenicity in glucose-6-phosphatase linked to glycogen storage disease type 1a

The paper uses AlphaMissense and experimental assays to analyze 78 G6PC1 variants, identifying stability and allosteric modulation as key pathogenic mechanisms.

Peptidic tryptophan halogenation by a promiscuous flavin-dependent enzyme

The paper characterizes ChlH, a flavin-dependent halogenase that halogenates internal and terminal tryptophan residues in diverse peptides and proteins, showing broad substrate promiscuity.

Defining unique structural features in the MAFA and MAFB transcription factors that control Insulin gene activity

The paper identifies structural features in MAFA and MAFB transcription factors that influence their DNA-binding and Insulin enhancer activity through structural and functional analysis.

Modeling Protein Conformations by Guiding AlphaFold2 with Distance Distributions. Application to Double Electron Electron Resonance (DEER) Spectroscopy.

The paper modifies AlphaFold2 to incorporate distance distributions from DEER spectroscopy, improving conformational prediction with fewer experimental constraints.

Alternating Access of a Neurotransmitter:Sodium Symporter Bacterial Homolog Determined from AlphaFold2 Ensembles and DEER Spectroscopy

This paper uses AlphaFold2 and DEER spectroscopy to study the conformational dynamics of MhsT, revealing energy landscapes and environmental effects on transporter function.

Modeling Alternate Conformations with Alphafold2 via Modification of the Multiple Sequence Alignment

This paper demonstrates how modifying multiple sequence alignments with in silico mutagenesis enables Alphafold2 to predict alternate protein conformations, expanding its utility for drug design and structural studies.