Kazufumi Honda

EV-Tracer detects and profiles lineage-specific extracellular vesicles in cancer-fibroblast co-cultures, revealing interferon-related RNA signatures associated with cell-cell contact.

This paper introduces a biosensor-based method for single-molecule enzyme activity profiling without enzyme-specific substrates, enabling absolute quantification of active enzyme molecules in cellular and circulating samples.

This study develops fluorogenic ProTide probes to profile carboxypeptidase activities, showing elevated levels in pancreatic cancer patients.

This paper introduces a programmable chemical strategy called SCCR for automated synthesis of protease-activatable molecules, demonstrating its application in generating fluorogenic probes and ADC linkers.

This study introduces a genome editing method to rapidly generate and test driver mutations in target genes, identifying novel resistance mutations in lung cancer.

This paper develops a single-molecule enzyme activity assay for serine hydrolases using activity-based probes and demonstrates its application in detecting active granzyme B as a biomarker of liver damage.

This paper introduces a liquid biopsy platform that detects early-stage pancreatic adenocarcinoma by analyzing single-molecule protease/peptidase activity in blood samples.

The paper identifies ACTN4-dependent ruffle-edge lamellipodia as a novel mode of cell migration using live-cell imaging and CLEM, showing their role in cancer cell invasion.