Mark A. Nakasone

This study develops a DIA-based workflow for comprehensive ubiquitinome profiling in targeted protein degradation, identifying over 40,000 ubiquitylated peptides.

The paper identifies LRRC58 as a substrate adaptor in a Cul2 E3 ligase complex that regulates CDO1 degradation in response to cysteine availability, preventing ferroptosis and neurodevelopmental defects.

The paper develops a photocrosslinking activity-based probe to study interactions between ubiquitin and RING E3 ligases, confirming existing structures and evaluating new models.

This paper develops a molecular glue-based PROTAC (MGPROTACs) to ubiquitinate the 14-3-3/Estrogen Receptor complex, demonstrating cooperative complex formation and selective ubiquitination through linker design.

This study shows that a monovalent MGD can recruit two E3 ligases redundantly to degrade SMARCA2/4, with tunable ligase preference through chemical and genetic modifications.

BromoCatch is a new self-labelling protein tag platform that enables rapid, specific, and stable protein labelling in live cells through covalent ligand engagement.

The paper solves cryo-EM structures of a degrader-mediated complex to reveal structural insights into target protein ubiquitination mechanisms.