Gopal P. Sapkota

This study develops a DIA-based workflow for comprehensive ubiquitinome profiling in targeted protein degradation, identifying over 40,000 ubiquitylated peptides.

This study shows targeted dephosphorylation of phospho-Tau at the endogenous level promotes its association with microtubules.

This study identifies the molecular mechanisms underlying the functional divergence of CK1 paralogs in the Wnt pathway, showing how CK1 and CK1δ/ε have distinct roles and affinities for substrates and complexes.

This study shows that molecular glue degraders targeting CK1 can co-degrade multiple SACK1 proteins, highlighting the role of protein complex context in targeted protein degradation.

The paper identifies mutations in SACK1G that disrupt its interaction with CK1α, using computational modeling and experimental validation.

This study identifies HMMR's role in stabilizing the SACK1D-CK1 complex at the mitotic spindle and shows how phosphorylation drives its degradation after mitosis.

This paper describes the engineering of a PROTAC that uses p97-directed proteolysis to achieve specific substrate degradation without relying on E3 ubiquitin ligases.

This study demonstrates targeted degradation of α-synuclein using a nanobody-guided system, preventing PFF-induced aggregation in neurons.

The paper shows that AMPK activation leads to dephosphorylation of TFEB's C-terminal sites, promoting its transcriptional activation, and that AMPK is not required for mTORC1-mediated TFEB activation.

This study generates fam83f knock-out zebrafish to investigate Fam83f's role in hatching and DNA damage response, finding impaired autophagy and increased radiation sensitivity.

This paper demonstrates the degradation of endogenous K-RAS and H-RAS using an AdPROM system, offering a new approach for targeting undruggable cancer proteins.