Michael A. Cianfrocco

This study shows that taccalonolide AJ stabilizes microtubules through a mechanism distinct from paclitaxel, promoting a compacted, post-GTP hydrolysis state that resists depolymerization.

CsMT provides a streamlined CryoSPARC workflow for cryo-EM reconstruction of microtubules, achieving high-resolution results with minimal manual intervention.

This study identifies KIFBP-regulated kinesins during neuronal development using proteomic and imaging approaches, revealing KIF5A and KIF18B as novel targets.

This study identifies structural switches in tubulin that Taxol exploits to stabilize microtubules by reverting them to a pre-hydrolysis state.

This study presents the cryo-EM structure of human ATAD2B, revealing its hexameric organization and providing insights into its ATPase activity and substrate coordination.

The paper investigates the accessibility of the α-tubulin C-terminal tail (Y-CTT) and finds it is regulated by microtubule lattice conformation through nucleotide state and MAP interactions.

This paper describes a laboratory's transition from X-ray crystallography to cryo-EM for studying ATAD2B, detailing challenges and strategies in sample preparation, data processing, and model building.

CryoSift automates 2D class selection in cryo-EM by using a CNN to assess particle quality, enabling high-throughput processing for high-resolution reconstructions.

Magellon provides a flexible and scalable cryo-EM platform with microservices architecture for data management, processing, and visualization.

This study compares the conformational flexibility of Drosophila and bovine tubulins and links it to differences in microtubule dynamic instability.

The paper shows that cargo adaptors bind myosin V through multiple sites, using a handhold mechanism to enhance motor-cargo interactions and regulation.

This paper uses generative adversarial networks to denoise cryo-EM data, improving structural information recovery from noisy images.

The paper develops cloud-based software tools for cryo-EM structure determination and atomic model refinement, reducing barriers for new users.