This study directly observes ATP-driven ubiquitin chain handling by Cdc48, revealing non-processive and processive modes of action and their role in ubiquitin unfolding and chain selection.

Nature Communications · May 4, 2026Peer-reviewedBiophysics

ATXN3 regulates lysosome regeneration after damage by targeting K48-K63-branched ubiquitin chains

Maike Reinders, Bojana Kravić, Pinki Gahlot, Sandra Koska, Johannes van den Boom, Nina Schulze, Sophie Levantovsky, Stefan Kleine, Markus Kaiser, Yogesh Kulathu, Christian Behrends, Hemmo H Meyer

This study shows ATXN3 helps restore lysosomal function after damage by targeting K48-K63-branched ubiquitin chains on regenerating lysosomes.

The EMBO Journal · Jul 29, 2025Peer-reviewedCell Biology

VCF1 is an unconventional p97/VCP cofactor promoting recognition of ubiquitylated p97-UFD1-NPL4 substrates

Ann Schirin Mirsanaye, Saskia Hoffmann, Melanie Bianca Weisser, Andreas Mund, Blanca Lopez Mendez, Dimitris Typas, Johannes van den Boom, Bente Benedict, Ivo Alexander Hendriks, Michael Lund Nielsen, Hemmo H Meyer, Julien P. Duxin, Guillermo Montoya, Niels Mailand

This paper identifies VCF1 as a novel p97 cofactor that enhances p97-UFD1-NPL4 interactions with ubiquitylated substrates, promoting proteasomal degradation.

bioRxiv · Jul 25, 2023 · PreprintMolecular Biology