Niels Mailand

ZATT/ZNF451 promotes release of stalled TOP2 cleavage complexes

This study identifies ZATT/ZNF451 as a key factor in resolving stalled TOP2 cleavage complexes without relying on TDP2, proteolysis, or DSB repair pathways.

mnDINO: Accurate and robust segmentation of micronuclei with vision transformer networks

mnDINO is a vision transformer model for accurate micronuclei segmentation in DNA-stained images, achieving high accuracy and generalization across cell lines and microscopes.

A proteome-wide dependency map of protein interaction motifs

The paper systematically assesses the function of short linear motifs (SLiMs) by mutating and screening them in human cells, identifying essential SLiMs for cell proliferation and uncovering novel classes and binding partners.

Targeted disruption of linkage-specific ubiquitylation reveals a key role of K29-linked ubiquitylation in epigenome integrity

This study uses a ubiquitin replacement strategy to show K29-linked ubiquitylation regulates SUV39H1 stability and H3K9me3 levels in human cells.

Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation

The paper identifies TOPORS as a SUMO-targeted ubiquitin ligase (STUbL) essential for SUMO-dependent DNA-protein crosslink repair and stress responses, showing its cooperative function with RNF4.

Profiling ubiquitin signaling with UBIMAX reveals DNA damage- and SCFβTRCP-dependent ubiquitylation of the actin-organizing protein Dbn1

The paper introduces UBIMAX, a method to profile ubiquitylation, and identifies Dbn1 as a novel DNA damage-induced ubiquitylation target regulated by SCFβTRCP.

VCF1 is an unconventional p97/VCP cofactor promoting recognition of ubiquitylated p97-UFD1-NPL4 substrates

This paper identifies VCF1 as a novel p97 cofactor that enhances p97-UFD1-NPL4 interactions with ubiquitylated substrates, promoting proteasomal degradation.