The paper shows ASPPs multimerize PP1 to form phosphatase hubs at cell-cell junctions, and mutant ASPPs disrupt this oligomerization, which can be rescued by forcing PP1 clustering.

PLoS Genetics · Oct 16, 2025Peer-reviewedCell Biology

NHR-23 and SPE-44 regulate distinct sets of genes during C. elegans spermatogenesis

James Matthew Ragle, Kayleigh N. Morrison, An A. Vo, Zoe E. Johnson, Javier Hernandez Lopez, Andreas Rechtsteiner, Diane C. Shakes, Jordan D. Ward

The paper identifies distinct gene regulation by NHR-23 and SPE-44 during C. elegans spermatogenesis and shows their combined depletion affects additional genes.

G3 Genes Genomes Genetics · Sep 22, 2022Peer-reviewedGenetics

The conserved molting/circadian rhythm regulator NHR-23/NR1F1 serves as an essential co-regulator of C. elegans spermatogenesis

James Matthew Ragle, Abigail L. Aita, Kayleigh N. Morrison, Raquel Martínez-Méndez, Hannah N. Saeger, Guinevere E. Ashley, Londen C. Johnson, Katherine Schubert, Diane C. Shakes, Jordan D. Ward

This study identifies NHR-23/NR1F1 as a critical regulator of spermatogenesis in C. elegans, functioning downstream of the sex determination pathway and independent of the SPE-44 network.

Development · Jan 1, 2020Peer-reviewedDevelopmental Biology