Laurence A. Florens

One Drosophila Ref(2)P polypeptide takes different structures in midgut and muscle, driving autophagy or mitochondria-associated degradation; deleting disordered region IDR2 switches muscle Ref(2)P to midgut state.

The paper uses an integrative approach to model the structure of an IDR-driven HDAC2 complex, revealing interactions not captured by AlphaFold alone.

The paper builds structural models of HDAC1/2-containing chromatin remodeling complexes using XL-MS and integrative modeling, revealing how the CTD IDR of HDAC1 folds into alpha helices.

This study identifies APR9 and KinesinA as essential components of the apical polar ring in Toxoplasma gondii, showing that their combined loss paralyzes the parasite and impairs key life cycle processes.

The paper shows that increasing translation initiation rates accelerates nucleation of aggregating proteins at polysomes, suggesting cotranslational nucleation influences proteome evolution.

The paper uses yeast aging to map proteome changes across age, revealing structural determinants of age-sensitive remodeling and providing a resource for aging research.

The paper uses CRISPR-RfxCas13d screening in zebrafish to identify Bckdk as a post-translational regulator of the maternal-to-zygotic transition, showing its role in ZGA and miR-430 processing.

The paper shows ASPPs multimerize PP1 to form phosphatase hubs at cell-cell junctions, and mutant ASPPs disrupt this oligomerization, which can be rescued by forcing PP1 clustering.

The paper identifies TFIID and SAGA complex subunits in C. albicans and shows that TAF12 paralogs form heterodimers through cotranslational assembly, ensuring specificity and stability of histone-like complexes.

The paper identifies distinct regions of SAP25 that recruit O-GlcNAc transferase, TET methylcytosine dioxygenases, and ubiquitin ligase/hydrolase activities to the Sin3/HDAC complex.

This study identifies DYRK1A's role in promoting mTORC1 activity by inhibiting TSC complex activity through phosphorylation of TSC2, and shows conservation in Drosophila.

This study identifies BB0346 as an essential LolA homolog in Borrelia burgdorferi that functions in a LolB-deficient lipoprotein sorting system, leading to mislocalization of inner membrane lipoproteins.

This study identifies FAM60A as a regulator of RNA splicing, showing it interacts with HDAC1 and spliceosome components, expanding its known role beyond the Sin3/HDAC complex.

The paper identifies two new proteins, Thc1 and Bmc1, that function with Pof8 in telomerase biogenesis by recognizing folded telomerase RNA and facilitating complex assembly.

This study shows that budding yeast adapts to long-term high temperature by reducing temperature-sensitive proteins, relocalizing the proteome, and altering protein conformations, which may confer new functions.

The paper identifies a novel DNA binding motif, HB1RE, associated with HOXB1-mediated gene repression in mouse ES cells.

This study uses decellularization, proteomics, and RNAi in planarians to identify ECM components essential for tissue homeostasis and regeneration.

The paper introduces SCAP, a method combining affinity purification, live cell imaging, and cross-linking mass spectrometry to build integrative structural models of protein complexes.

The paper investigates how paralog switching affects Sin3 complex interaction networks in humans, revealing shared and distinct interactions between SIN3A and SIN3B.

The paper maps the WDR76 interactome using AP-MS and identifies key interactions with the CCT complex and other proteins, validating interactions with GAN and SIRT1.

The paper introduces TopS, a method to score protein interactions in proteomic datasets, revealing direct interactions and modules in human and yeast networks.

This paper identifies DYRK1A's role in DNA repair by regulating RNF169-mediated 53BP1 recruitment to DSB sites, with implications for DNA damage therapy response.

The paper identifies a novel protein complex, WHHERE, that regulates retinoic acid signaling and embryonic symmetry by controlling histone modifications and RNA Polymerase II recruitment.

The paper identifies combinatorial binding of Hoxa1 and TALE proteins on genome-wide targets and reveals their cross-regulatory interactions.