Guinevere E. Ashley

A conserved chronobiological complex times C. elegans development

This study identifies a conserved signaling module involving LIN-42 and KIN-20 that regulates molting timing in C. elegans, mirroring the mammalian PER-CK1 complex.

Multiscale patterning of a model apical extracellular matrix revealed by systematic endogenous protein tagging

The paper develops a toolkit for fluorescently tagging apical extracellular matrix components in C. elegans to study their molecular architecture and spatial organization.

Regulation of the circadian clock in C. elegans by clock gene homologs kin-20 and lin-42

This study investigates the role of lin-42 and kin-20 in the circadian clock of C. elegans using a bioluminescence reporter, showing they regulate the period length through neuronal cells.

Expanding the Caenorhabditis elegans auxin-inducible degron system toolkit with internal expression and degradation controls and improved modular constructs for CRISPR/Cas9-mediated genome editing

The paper introduces new tools for the auxin-inducible degron system in C. elegans, enabling conditional protein depletion through CRISPR/Cas9-mediated genome editing.

The conserved molting/circadian rhythm regulator NHR-23/NR1F1 serves as an essential co-regulator of C. elegans spermatogenesis

This study identifies NHR-23/NR1F1 as a critical regulator of spermatogenesis in C. elegans, functioning downstream of the sex determination pathway and independent of the SPE-44 network.

Rapid degradation of C. elegans proteins at single-cell resolution with a synthetic auxin

This paper improves the AID system in C. elegans for single-cell protein degradation using NAA and demonstrates its application in developmental studies.