This study defines DNAJA2 co-chaperone client selectivity using pulse-SILAC and BioID to reveal distinct protein pools associated with DNAJA2 based on structural and physicochemical properties.

bioRxiv · Jul 17, 2026 · PreprintBiochemistry

The co-chaperone DNAJA2 buffers proteasomal degradation of cytosolic proteins with missense mutations

Heather A. Baker, Jonathan P. Bernardini, Veronika Csizmók, Angel M. Madero, Shriya Kamat, Hailey Eng, Jessica Lacoste, Faith Au Yeung, Sophie A. Comyn, Elizabeth Hui, Gaetano Calabrese, Brian Raught, Mikko Taipale, Thibault Mayor

The paper identifies cytosolic mutant proteins degraded by the proteasome and shows DNAJA2 stabilizes some mutants while being dispensable for wild-type proteins.

Journal of Cell Science · Dec 2, 2024Peer-reviewedCell Biology

Tsa1 is the dominant peroxide scavenger and a source of H2O2-dependent GSSG production in yeast

Jannik Zimmermann, Lukas Lang, Gaetano Calabrese, Hugo Laporte, Prince Saforo Amponsah, Christoph Michalk, Tobias Sukmann, Julian Oestreicher, Anja Tursch, Esra Peker, Theresa N E Owusu, Matthias Weith, Letícia Prates Roma, Marcel Deponte, Jan Riemer, Bruce Morgan

This study identifies Tsa1 as the primary peroxide scavenger in yeast and shows it can generate GSSG under H2O2 conditions.

Free Radical Biology and Medicine · Nov 6, 2024Peer-reviewedBiochemistry