autoFISH provides a modular toolbox for automated smFISH experiments, including equipment plans, software, protocols, and analysis workflows validated on cell lines and tissues.

bioRxiv · Nov 14, 2024 · PreprintMolecular Biology

Tsa1 is the dominant peroxide scavenger and a source of H2O2-dependent GSSG production in yeast

Jannik Zimmermann, Lukas Lang, Gaetano Calabrese, Hugo Laporte, Prince Saforo Amponsah, Christoph Michalk, Tobias Sukmann, Julian Oestreicher, Anja Tursch, Esra Peker, Theresa N E Owusu, Matthias Weith, Letícia Prates Roma, Marcel Deponte, Jan Riemer, Bruce Morgan

This study identifies Tsa1 as the primary peroxide scavenger in yeast and shows it can generate GSSG under H2O2 conditions.

Free Radical Biology and Medicine · Nov 6, 2024Peer-reviewedBiochemistry

Radiotherapy triggers pro-angiogenic signaling in human lung

Juliette Soulier, Sandra Curras-Alonso, Maxime Dubail, Hugo Laporte, Mallick Ayan, Chloé Lafouasse, Delphine Colin, Jean-François Côté, Jérôme Didier, Christelle Pouliquen, Abdelali Benali, Marco Alifano, Catherine Durdux, Diane Damotte, Marine Lefèvre, , Charles Fouillade

This study uses single-cell RNA sequencing to show that radiotherapy induces pro-angiogenic signaling in human lung tissue, including upregulation of VEGFA and changes in capillary and macrophage populations.

bioRxiv · Oct 11, 2024 · PreprintCancer Biology

A point cloud segmentation framework for image-based spatial transcriptomics

Thomas Defard, Hugo Laporte, Mallick Ayan, Juliette Soulier, Sandra Curras-Alonso, Christian Weber, Florian Massip, Arturo Londoño‐Vallejo, Charles Fouillade, Florian Mueller, Thomas Walter

This paper introduces ComSeg, a segmentation algorithm for image-based spatial transcriptomics that outperforms existing methods in cell type calling and handles complex tissue structures.

Communications Biology · Jul 6, 2024Peer-reviewedBioinformatics