This paper develops a virus-based system for transgene- and tissue culture-free heritable genome editing in tomato, achieving somatic editing and mutant progeny without transgenic materials.

Proceedings of the National Academy of Sciences · Jun 4, 2026Peer-reviewedPlant Biology

Efficient transgene-free multiplexed genome editing via viral delivery of an engineered TnpB.

Trevor J. Weiss, Maris Kamalu, Honglue Shi, Gabriel Wirnowski, Alice Ingelsson, Jasmine Amerasekera, Kamakshi Vohra, Marena I. Trinidad, Z. M. Li, Emily dos Santos Galante Freitas, Noah Steinmetz, Charlie Ambrose, Kerry Chen, Jennifer A. Doudna, Steven E. Jacobsen

The paper improves a virus-mediated genome editing method in plants by using multiple gRNAs and an enhanced TnpB variant for efficient multiplexed editing.

Plant Biotechnology Journal · Mar 17, 2026Peer-reviewedPlant Biology

Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis

Trevor J. Weiss, Maris Kamalu, Honglue Shi, Zheng Li, Jasmine Amerasekera, Zhenhui Zhong, Benjamin A. Adler, Michelle M Song, Kamakshi Vohra, Gabriel Wirnowski, Sidharth Chitkara, Charlie Ambrose, Noah Steinmetz, Ananya Sridharan, Diego Sahagun, , Steven E. Jacobsen

This paper engineers a plant virus to deliver a compact CRISPR-Cas system for transgene-free germline editing in Arabidopsis.

Nature Plants · Apr 22, 2025Peer-reviewedPlant Biology

GeneWeld: a method for efficient targeted integration directed by short homology

Wesley A. Wierson, Jordan M. Welker, Maira P. Almeida, Carla M. Mann, Dennis A. Webster, Melanie E. Torrie, Trevor J. Weiss, Macy Vollbrecht, Merrina Lan, Kenna C McKeighan, Jacklyn Levey, Zhitao Ming, Alec Wehmeier, Christopher S Mikelson, Jeffrey Haltom, , Jeffrey J. Essner

GeneWeld enables efficient and precise targeted integration in multiple species using CRISPR/Cas9 with minimal homology arms.

bioRxiv · May 25, 2019 · PreprintGenetics