Steven E. Jacobsen

This study identifies RRsS1 as a dual-binding scaffold that stabilizes Pol IV transcription complexes to enhance siRNA biogenesis in plant reproduction.

The paper identifies SNPC-1.2 as a female-specific piRNA transcription factor in C. elegans, revealing how gene duplication and functional specialization generate sex-specific piRNA expression.

This study identifies MORC and MOM1 as spatial regulators of RNA Polymerase V chromatin positioning, which influences DNA methylation landscapes in plants.

The paper identifies J-domain proteins as mediators of PKL recruitment to gene promoters, enabling H3.3 deposition and influencing gene expression through interactions with transcription factors and ATRX.

SunTag-NOVA, a SunTag-MQ1v editor adding TRBIP1 and an oligomerizing CHLAMY domain, methylates and silences FWA, FT and TMM in Arabidopsis without the genome-wide off-target methylation seen with TRBIP1 alone.

Ymu1 TnpB unwinds DNA via a long-lived partially unwound intermediate before a rarely stable open state; the WFR triple variant stabilizes both, boosting plant editing.

This study identifies DAF-16/FOXO's role in repressing let-7 microRNA and NHR-23 to maintain multipotency during dauer in C. elegans.

The paper designs active RNA-guided nucleases using AI and structure-guided methods, validated by cryo-EM and functional testing across species.

This paper identifies a fungal natural product, MDD, as a novel cellulose biosynthesis inhibitor that targets CESA1 and disrupts cellulose synthase complexes, enabling the development of multi-drug resistant crop varieties.

The paper tests two CRISPR-based epigenome editing tools in cassava to induce DNA methylation at susceptibility gene promoters, reducing pathogen infection and showing inheritance patterns.

This paper develops a virus-based system for transgene- and tissue culture-free heritable genome editing in tomato, achieving somatic editing and mutant progeny without transgenic materials.

The paper improves a virus-mediated genome editing method in plants by using multiple gRNAs and an enhanced TnpB variant for efficient multiplexed editing.

The paper shows that multiple redundant mechanisms account for most gene silencing downstream of DNA methylation, with loss of these pathways causing widespread derepression and genomic changes.

The paper uses CRISPR interference to methylate promoters of eIF4E genes in cassava, reducing gene expression and disease susceptibility.

The paper reveals an antagonistic epigenetic mechanism in Arabidopsis pollen where MBD5/6 repress methylated loci while MBD7 promotes demethylation and transcriptional activation.

This study uses CRISPR to target H3K4me3 methyltransferases in Arabidopsis, showing activation of gene expression and increased meiotic crossover recombination near centromeres.

The paper shows CDCA7A and CDCA7B maintain CG methylation in centromeric heterochromatin by remodeling H1-containing nucleosomes, with H1 having an inhibitory role.

The paper identifies Arabidopsis REM transcription factors and GDE1 as key regulators of DNA methylation by recruiting RNA Polymerase IV to generate 24nt-siRNAs that direct methylation to specific genomic loci.

This paper engineers a plant virus to deliver a compact CRISPR-Cas system for transgene-free germline editing in Arabidopsis.

This study shows that MORC3 is essential for T cell development in the thymus, as its loss causes developmental arrest and altered chromatin accessibility in DN1 cells.

The paper shows MORC-1 overexpression in csr-1 mutants drives gene downregulation by altering chromatin states, revealing its role in CSR-1-mediated gene licensing.

The paper identifies that unstable transcripts are rare in plants and often originate from promoters, challenging the use of genome annotations to infer enhancers.

This study identifies MBD2 as a methyl reader that silences transposable elements during male gametogenesis by acting downstream of DNA methylation.

The paper traces the evolutionary origin of eukaryotic RNA-guided endonucleases, showing they evolved from a specific clade of bacterial IS607 TnpB enzymes.

The paper shows that Drosophila males produce Su(Ste) piRNAs through 5'-to-3' phased biogenesis triggered by maternally deposited piRNAs, and this process is bypassed when mothers are XXY.

The paper uses single-nucleus RNA-seq to show that MBD5, MBD6, and SILENZIO are critical for TE silencing in pollen vegetative cells during epigenetic reprogramming.

This study shows that loss of H3.1K27me1 in Arabidopsis leads to resistance against Geminivirus by sequestering DNA repair proteins to defense genes and rDNA, reducing viral replication.

The paper identifies an OGT-TET3 complex that regulates 5mC and 5hmC levels and XCI in female mouse embryonic stem cells.

This study shows that H2A.W and H1 co-regulate heterochromatin accessibility and DNA methylation in flowering plants by modulating H1 deposition and non-CG methylation.

The paper identifies SNPC-1.3 as a male-specific transcription factor that regulates piRNA expression in C. elegans and shows its sex-specific regulation by TRA-1.