Cell-free prototyping enables implementation of optimized reverse beta-oxidation pathways in heterotrophic and autotrophic bacteria
Bastian Vögeli, Luca Schulz, Shivani Garg, Katia Tarasava, James M. Clomburg, Seung Hwan Lee, Aislinn Gonnot, Elamar Hakim Moully, Blaise R. Kimmel, Loan Tran, Hunter Zeleznik, Steven D. Brown, Séan Dennis Simpson, Milan Mrksich, Ashty S. Karim, , Michael C. Jewett
The paper optimizes and implements reverse beta-oxidation pathways in bacteria for carbon-negative synthesis of C4-C6 acids and alcohols, achieving high product titers in engineered strains.
Nature Communications · Jun 1, 2022Peer-reviewedSynthetic Biology