Giuseppe Vicidomini

BrightEyes-Scope: A Reference Implementation for Image Scanning Microscopy with SPAD Array Detection

BrightEyes-Scope provides a reference implementation for SPAD array-based image scanning microscopy, offering guidelines for system construction and image reconstruction to improve super-resolution imaging.

Independent noise realizations enable morphologically agnostic image reconstruction in single-photon-sensitive microscopy

This paper introduces a morphology-agnostic regularization framework for image deconvolution in single-photon-sensitive microscopy, eliminating semi-convergence and improving reconstruction quality.

BrightEyes-FFS: an open-source platform for comprehensive analysis of fluorescence fluctuation spectroscopy experiments with small detector arrays

BrightEyes-FFS provides an open-source platform for analyzing fluorescence fluctuation spectroscopy data with small detector arrays, offering tools for data processing, correlation analysis, and model fitting.

Mapping the diffusional landscape of short NEAT1 in living cells

This study visualizes and characterizes the diffusivity of NEAT1-1 in live cells, revealing its presence in a distinct diffusive state within the nucleoplasm.

Array Detection Enables Large Localization Range for Simple and Robust MINFLUX

This paper introduces ISM-FLUX, a MINFLUX implementation using a SPAD array detector, achieving larger localization range and simpler architecture.

Open-Source 3D Active Sample Stabilization for Fluorescence Microscopy

This paper introduces an open-source 3D active stabilization system for fluorescence microscopy to maintain sample stability during long imaging sessions.

Wavefront estimation through structured detection in laser scanning microscopy

This paper introduces a convolutional neural network for aberration correction in laser scanning microscopy using detector array data, achieving high-resolution imaging with minimal sample stress.

The role of RNA in the nanoscale organization of alpha-synuclein phase separation

This study investigates how RNA affects alpha-synuclein phase separation, showing RNA induces morphological changes and promotes liquid-to-solid transition.

ALS-associated FUS mutation reshapes the RNA and protein composition of Stress Granules.

This study examines how ALS-associated FUS mutations alter the RNA and protein composition of Stress Granules in neural cells, revealing molecular changes linked to neurodegeneration.

M6A reduction relieves FUS-associated ALS granules

The paper shows that reducing m6A content via METTL3 knock-down decreases FUS-associated ALS granules in cell models and patient-derived fibroblasts.

Fourier ring correlation simplifies image restoration in fluorescence microscopy

This paper introduces blind image restoration methods using Fourier ring correlation (FRC) for de-noising and deconvolution in fluorescence microscopy, showing improved performance in 2D and 3D images.