Sachiko Tamura

Machine learning-assisted Repli-Histo labeling reveals distinct transcription-dependent constraints on chromatin motion in living cells

The paper uses machine learning to classify chromatin classes and shows how different transcription systems affect nucleosome motion in distinct chromatin regions.

Average local nucleosome motion remains nearly constant during interphase in living human cells

The paper measures local nucleosome motion in living human cells during interphase and finds it remains nearly constant, with changes observed under specific perturbations.

Cohesin prevents local mixing of condensed euchromatic domains in living human cells

This study shows cohesin maintains condensed euchromatic domains in human cells by preventing local mixing, which is crucial for transcriptional insulation.

Linker histone H1 functions as a liquid-like glue to organize chromatin in living human cells

This study shows that linker histone H1 acts as a liquid-like glue in chromatin domains, enabling dynamic interactions that support DNA transactions in human cells.

Replication-dependent histone (Repli-Histo) labeling dissects the physical properties of euchromatin/heterochromatin in living human cells.

The paper develops Repli-Histo labeling to study nucleosome motion in euchromatin and heterochromatin, finding that euchromatic regions exhibit greater motion and influence replication timing.

Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo

This study uses electron cryotomography to show that budding yeast chromatin lacks 30-nm fibers and is in an open configuration, suggesting transcription regulation through local nucleosome interactions.