Masato T. Kanemaki

Firrm functions as a multiprotein interaction platform that coordinates Mcm8-9-Hrob recruitment and Fignl1-mediated Rad51 unloading

The paper identifies Firrm as a platform coordinating Mcm8-9-Hrob recruitment and Fignl1-mediated Rad51 unloading during homologous recombination.

Human FACT complex coordinates cohesin and transcription on chromatin

This study shows that the FACT complex regulates cohesin dynamics on chromatin, facilitating loop extrusion and preventing cohesin accumulation in transcribed regions.

Average local nucleosome motion remains nearly constant during interphase in living human cells

The paper measures local nucleosome motion in living human cells during interphase and finds it remains nearly constant, with changes observed under specific perturbations.

MCM10 and RECQL4 have cooperative and redundant roles in activating the CMG helicase during the replication initiation

This study shows MCM10 and RECQL4 work together and can replace each other in activating the CMG helicase during DNA replication initiation.

Cohesin prevents local mixing of condensed euchromatic domains in living human cells

This study shows cohesin maintains condensed euchromatic domains in human cells by preventing local mixing, which is crucial for transcriptional insulation.

Cohesin acts as a transcriptional gatekeeper by restraining pause-release to promote processive elongation

This study shows cohesin regulates multiple transcription steps, with loss leading to reduced induction but minimal steady-state expression changes.

Highly active chromosome regions preferentially associate with two perispeckle networks that partition the interchromatin space

The paper identifies two perispeckle networks that partition interchromatin space, showing differential gene expression associations compared to nuclear speckles.

Cohesin residence time gates 3D genome response to histone hyperacetylation

This study shows that cohesin residence time, not just its presence, determines how histone hyperacetylation affects 3D genome structure.

m1A58 governs two steps of human initiator-methionine tRNA metabolism: maturation and XRN2-mediated decay

This study shows m1A58 regulates tRNA maturation and XRN2-mediated decay in human cells, maintaining tRNA homeostasis.

BRCA2 loss triggers a downward spiral of genomic instability via ROS-dependent metabolic collapse

This paper shows BRCA2 loss causes genomic instability through ROS-dependent metabolic collapse, highlighting the role of glutathione metabolism and PARP inhibitors in cell death.

Gene transcription and chromatin packing domains form a self-organizing system

The paper shows that Pol-II regulates chromatin packing domains through transcriptional loops, linking RNA synthesis to genome organization and gene expression.

Cohesin drives chromatin scanning during the RAD51-mediated homology search.

The paper shows cohesin-driven chromatin loop extrusion facilitates homology search during RAD51-mediated DNA repair in mammalian cells.

The mammalian rapid tRNA decay pathway is critical for N⁷-methylguanosine-hypomodified tRNA degradation under physiological conditions

The paper shows that XRN2-dependent decay of mG-hypomodified tRNAs occurs under physiological conditions and is conserved across species.

The human RIF1-Long isoform interacts with BRCA1 to promote recombinational fork repair under DNA replication stress

The paper identifies RIF1-Long as a BRCA1 binding partner that enhances RAD51-dependent repair during DNA replication stress.

DNA methylation protects cancer cells against senescence

The paper shows that reduced DNA methylation in cancer cells leads to senescence without DNA damage, involving p21 and cGAS, and demonstrates this in vivo.

A single-chain antibody-based AID2 system for conditional degradation of GFP-tagged and untagged proteins

This paper develops a scAb-AID2 system for conditional protein degradation without requiring degron tags, demonstrating its effectiveness in human and C. elegans cells.

The depletion of TRAIP results in the retention of PCNA on chromatin during mitosis, leads to inhibiting DNA replication initiation.

This study shows that TRAIP depletion causes PCNA retention on chromatin during mitosis, leading to reduced Cdt1 levels and impaired S phase entry.

Nuclear blebs are associated with destabilized chromatin packing domains

The paper investigates how nuclear blebs alter chromatin packing domains, finding fragmentation of nanoscopic heterochromatin domains within blebs.

mRNA expression is co-regulated by non-nucleolar RNA polymerase I

The paper shows that RNA polymerase I co-regulates chromatin domains and mRNA synthesis with RNA polymerase II, affecting genome connectivity and gene expression.

Combination of AID2 and BromoTag expands the utility of degron-based protein knockdowns

The paper combines AID2 and BromoTag degron systems to enhance protein depletion, demonstrating their effectiveness in suppressing DNA replication by co-depleting ORC1 and CDC6.

Cohesin-mediated 3D contacts tune enhancer-promoter regulation

This study uses CRUDO to show that cohesin and 3D contacts influence enhancer-promoter regulation, with distal enhancers showing stronger cohesin dependence.

Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells

This study shows UHRF1 has non-canonical roles beyond DNMT1 activation in maintaining DNA methylation homeostasis in cancer cells.

Cohesin regulates promoter-proximal pausing of RNA Polymerase II by limiting recruitment of super elongation complex

This study shows cohesin limits super elongation complex recruitment to promoters, thereby regulating RNA Polymerase II pausing and gene expression.

Chromatin packing domains persist after RAD21 depletion in 3D.

The paper shows that RAD21 depletion reduces but does not eliminate chromatin packing domains in electron microscopy, suggesting non-cohesin mechanisms largely control 3D structure.

Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos

This study identifies a specialized Ran-GTP-driven spindle structure in medaka embryos that ensures accurate chromosome segregation during early cleavage divisions.

High-throughput Oligopaint screen identifies druggable regulators of genome folding

The paper develops HiDRO, a high-throughput imaging pipeline, to screen the human druggable genome and identifies >300 factors regulating chromatin folding, including GSK3A as a regulator of TAD interactions.

Global landscape of replicative DNA polymerase usage in the human genome

The paper uses Pu-seq to map DNA polymerase usage in human cells, revealing Polε synthesizes the leading strand and Pol contributes to lagging strand synthesis, and links transcription to polymerase uncoupling and chromosomal instability.

Cohesin-mediated loop anchors confine the location of human replication origins

This study shows that cohesin-mediated loops in G1 phase determine the genomic location of replication origins in S phase by anchoring replication initiation zones.

MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells

This study identifies MCMBP as essential for MCM2-7 hexamer assembly in human cells, showing its role in DNA replication and its potential as a target for p53-deficient cancers.

Replication timing maintains the global epigenetic state in human cells.

This study shows that replication timing (RT) is essential for maintaining the global epigenetic state and genome compartmentalization in human cells, with RIF1 playing a central role in this process.