Kelly E. Stecker

The paper benchmarks biotin enrichment in proximity labeling proteomics, showing that higher biotinylation improves specificity while lower levels increase background noise.

This study identifies TSPAN3 as a marker of a distinct secretory multivesicular body population with unique trafficking and EV composition compared to CD63-positive compartments.

The paper investigates pathways controlling microtubule density in interphase cells by disrupting CAMSAP and γ-TuRC pathways, revealing redundant activation mechanisms.

This study investigates how Katanin, CAMSAPs, WDR47, and kinesin-13 regulate microtubule abundance and minus-end dynamics through in vitro reconstitution.

This study shows taxanes induce conformational changes in microtubules, releasing GEF-H1 and activating RhoA signaling, leading to actin remodeling.

EVtrace uses SILAC to trace EV-associated proteins in recipient cells, showing non-stochastic targeting of EV subpopulations to specific cell types.

The paper identifies GDF15 as a negative regulator of necroptotic cell death by directly interacting with RIPK3, enhancing necroptosis in tumor cells.

This paper introduces a SILAC-TMT-RTS method to study proteome changes in T cell-cancer cell co-cultures, revealing mechanisms of differential sensitivity to T cell killing.

This study shows that inhibiting TBK1/IKKe-mediated RIPK1 phosphorylation increases tumor sensitivity to immune cell killing by disrupting TNF signaling.

The paper investigates the interactions of ciliary tip proteins and their role in microtubule dynamics, finding that they form structures that slow and processively elongate microtubules.

This study uses engineered protein probes to track DNA damage dynamics and identify novel DNA damage response factors in living cells and animals.