Stephen E. Kearsey

This study shows that cytoplasmic localization of RNR is essential for genome stability, as nuclear localization causes DNA replication defects and increased mutagenesis in fission yeast.

The paper measures mutation rates in S. pombe using canavanine resistance and finds that spontaneous mutants predominantly have an R175C mutation in the any1 gene, which confers resistance through altered arginine transport and cell morphology.

The paper shows that inherited broken chromosomes in S. pombe undergo cycles of replication and processing, leading to chromosomal instability and rearrangements.

The paper shows that disrupting dNTP homeostasis is synthetically lethal with CDK-induced replication stress in fission yeast, leading to genome instability and cell death.