Ryan Kelly

This study establishes quality control standards for single-cell proteomics through inter-laboratory benchmarking, identifying software and technical factors affecting data reliability.

This paper introduces a low-cost proteomics approach achieving deep quantitative proteome profiling at ~$10 per sample using specific mass spectrometers.

This study identifies CMG2-actin interaction as essential for growth factor-induced chemotaxis in endothelial cells, showing that CMG2 binds F-actin to enable directional migration.

PSMtags enhance peptide detection and sequence identification in proteomics, enabling 9-plexDIA and significantly increasing sample throughput.

The paper identifies a role for oocyte mitochondrial electron transport chain remodeling in linking maternal environment to offspring metabolism and survival.

This paper introduces AI-TMT, a method enhancing single-cell proteomics sensitivity by improving reporter ion generation, resulting in higher signal and faster analysis.

This paper introduces TIFF, a method enhancing single-cell proteomics sensitivity and accuracy by filtering background ions and using 3D MS1 feature matching, achieving deep proteome analysis in single cells.

This study examines how peptide fragmentation spectra differ between single cell and bulk proteomics, identifying key changes that affect peptide identification.

This study benchmarks peptide spectrum match identification tools for single cell proteomics and finds MetaMorpheus performs best at 1% FDR, with post-processing ML improving results by up to 40%.

This study investigates proteomic changes in CLL during the asymptomatic stage using small sample sizes and highlights the need for larger control populations sampled over time.

The paper calculates the sample size requirements for detecting proteome dynamics in single cell proteomics time course experiments.

This paper introduces an ultrasensitive single-cell proteomics workflow that identifies over 1000 protein groups per mammalian cell using advanced mass spectrometry techniques.